摘要
利用GLM模型对12个表达序列标签(expressed sequence tag,EST)标记的基因型与鲤耐低温性状进行相关性分析,然后使用OneMap软件将这些EST标记进行连锁定位研究,并通过Blast x搜索引擎对这些候选EST进行注释。结果显示,EST标记CC009(P<0.05)和CC115(P<0.01)与鲤鱼耐低温性状显著相关;12个EST标记中有8个标记分别连锁定位到6个连锁群中,其中与耐低温相关的CC009和CC115分别定位到鲤连锁图谱的第38号连锁群和第2号连锁群;蛋白质数据库同源性比对发现,CC009与斑马鱼(Danio rerio)尿嘧啶激酶1(uridine-cytidine kinaseI)的同源性高达94%;而CC115为原绿球藻(Prochlorococcus marinus str)的假定糖基转移酶(putative glycosyl transferase)的同源性为56%。
Expressed sequence tags （ESTs） are parts of complementary DNAs （cDNAs） with certain gene function.It provides us more information than other neutral markers. The association of EST markers with phenotypes can increase our understanding of the biochemical pathways and mechanisms affecting economically important traits. In this study, 12 candidate EST markers isolated from the cold-induced brain cDNA of common carp （Cyprinus carpio L.） were conducted the correlation analysis of marker and cold tolerance trait of common carp using GLM model of SPSS 17.0 software firstly, then tried to locate them in the genetic linkage map using OneMap software. As a result, eight out of 12 candidate EST makers were separately located in six linkage groups, in which marker CC009（P0.05） and CC115 （P0.01） were associated with cold tolerance and mapped to LG38 and LG2, respectivey. Homology identity alignments showed that marker CC009 was highly homologous to the known Uridine-cytidine kinase I of Danio rerio with an identity of 94%, and marker CC115 was lowly homologous to the putative glycosyl transferase of Prochlorococcus marinus with an identity of 56%.
基金
国家重点基础研究发展计划"973"(2010CB126305)
农业部水产生物技术重点开放实验室(2009NYBZS-05)
中央级公益性科研院所基本科研业务费专项资金项目(2009HSYZX-SJ-05)
关键词
鲤鱼
表达序列标签
耐低温
相关分析
连锁图谱
Common carp
Expressed sequence tag
Cold tolerance
Correlation analysis
Linkage map
作者简介
第一作者简介：常玉梅，女，助理研究员，博士，研究方向：鱼类分子生物学与基因工程育种。E—mail：ymchang@flshbreeding．org
通讯作者（Corresponding author）, E-mail： lqliang@fishbreeding.org
