摘要
基于利多卡因对联吡啶钌在铂电极上的电致发光信号有增敏作用,建立了一种测定利多卡因的毛细管电泳-电化学发光分析方法.讨论了磷酸盐缓冲液pH值、浓度、分离电压、检测电位等实验参数对利多卡因分离检测的影响.在优化的实验条件下,利多卡因在1.5～740μmol/L内呈良好线性,检出限为0.1μmol/L.应用此法测定了尿液中利多卡因的含量,回收率为90%～93.5%.
A rapid, simple and sensitive method was developed for the determination of lidocaine by capillary electrophoresis with electrochemiluminescence （ECL） detection, which was based on lidocaine enhanced the ECI. intensity of tris （2,2＇-bipyridyl） ruthenium （ Ⅱ ） in PBS medium. Sample solution was injected to separation capillary of 40 cm length （25 μm i. d. ） by electrokinetic injection for 10 s at 10 kV. Under the optimized conditions, the linear range from 1.5 to 740 μmol/L for lidocaine and detection limit （S/N = 3） of 0.1 μmol/L were achieved. The RSD of ECL intensity and the migration time for five continuous injections of 3.7 μmol/L were 4.0% and 0.7%, respectively. This method was successfully applied to lidocaine assay in urine samples with the recovery of 90%-93.5%.
出处
《河北大学学报（自然科学版）》
CAS
北大核心
2010年第6期652-656,共5页
Journal of Hebei University(Natural Science Edition)
基金
教育部博士点学科基金资助项目(20050075003)
河北省自然科学基金资助项目(B2008000583)
关键词
毛细管电泳
电化学发光
利多卡因
capillary electrophoresis
electrochemiluminescence
lidocaine
作者简介
孙汉文（1945-），男，河北魏县人，河北大学教授，博士生导师，主要从事光谱学分析、分离科学技术、环境分析化学与农兽药残留分析研究．
