摘要
[目的]观察通腑汤对急性肺损伤大鼠肺组织bFGF影响,探讨通腑汤对急性肺损伤的作用机制,为"肺与大肠相表里"理论提供实验依据。[方法]以Wistar大鼠为实验研究对象,将180只大鼠随机分为六组,即:正常对照组、模型组、地塞米松组、中药低剂量组、中药中剂量组、中药高剂量组,每组30只。经尾静脉注射LPS复制ALI模型,半小时后中药各剂量组灌胃、地塞米松组腹腔注射治疗,分别于治疗后第1、2、6h,每组每次处死10只大鼠。用ELISA法同步检测肺组织中bFGF的含量。[结果]ALI的肺组织中:模型组的bFGF含量明显高于空白对照组,有统计学意义(P<0.05);地塞米松组、中药低剂量组、中药中剂量组、中药高剂量组的bFGF含量明显低于模型组,有统计学意义(P<0.05);中药低剂量组的bFGF含量高于地塞米松组,有统计学意义(P<0.05);中药中剂量组、中药高剂量组的bFGF含量与地塞米松组无差异,无统计学意义(P>0.05);中药中剂量组、中药高剂量组的bFGF含量均低于中药低剂量组,有统计学意义(P<0.05);中药高剂量组的bFGF含量与中药中剂量组无差异,无统计学意义(P>0.05)。[结论]通腑汤可以降低ALI大鼠肺组织的bFGF含量,各剂量组中以中剂量、高剂量组疗效最佳。
[ Objective]To observe the effect on bFGF in ALI rat lung, and to approach the mechanism of Tongfu Decoction anti -ALI, so to provide experimental base on the lung and the large intestine being interior -exteriorly related theory. [Method] 180 Wistar rats were divided into 6 groups randomly： the normal control group, the model group, hexadecadrol group, Chinese herbal medicine low - dose group, medium - dose group, and high -dose group, 30 rats each group. Copying ALI model by caudal vein injecting LPS, giving respectively the Chinese herbal medicine intragastric administration and Dexamethasone intraperitoneal injection half an hour later, and executing rats in 1,2,6 hour after treatment respectively, 10 rats each time in each group. We observed lung histomorphology changes, detected bFGF content in lung by ELISA method synchro- nously. [ Result] In ALl lung： bFGF contents in the model group were higher than the normal control group （P 〈0.05） ; contents in hexadecadrol group and Chinese herbal medicine groups were lower than the model group（P 〈 0.05） ; contents in Chinese herbal medicine low -dose group were higher than hexadecadrol group （P 〈 0.05） ;Chinese herbal medicine medium -dose group^high -dose group and hexadecadrol group had no statistical significance （ P 〉 0.05 ） ; contents in Chinese herbal medicine medium - dose group and high - dose group showed lower than low -- dose group （ P 〈 0.05 ） ; Chinese herbal medicine medium - dose group and high - dose group had no statistical significance （ P 〉 0.05 ）. [ Conclusions ] Tongfu Decoction could decrease bFGF in lung of ALI rats, medium - dose and high - dose group were better.
出处
《实用中医内科杂志》
2010年第11期19-21,共3页
Journal of Practical Traditional Chinese Internal Medicine
基金
黑龙江省中医管理局项目
