摘要
目的探讨叶酸对高同型半胱氨酸血症(HHcy)诱发大鼠动脉粥样硬化的干预效果。方法健康8周龄雄性SD大鼠26只,体重(200±20)g,适应性喂养一周后随机分为对照组(6只)、模型组(10只)和叶酸干预组(10只)。对照组给予普通饲料喂养,模型组在普通饲料喂养的基础上加2%的蛋氨酸,叶酸干预组在普通饲料的基础上加2%的蛋氨酸及0.0003%叶酸。12周后,心脏取血测定血浆同型半胱氨酸(Hcy)浓度,取胸主动脉进行电镜观察和苏木素-伊红(HE)染色、油红O染色。结果模型组血浆Hcy水平明显高于对照组(P<0.01),叶酸干预组Hcy水平明显低于模型组(P<0.01)。电镜观察和HE、油红O染色均可见模型组主动脉内皮下脂质和泡沫细胞沉积,亦即动脉粥样硬化脂纹形成。叶酸干预组与对照组相比无明显改变。结论HHcy可诱发大鼠主动脉粥样硬化早期病变的形成,叶酸干预可有效预防HHcy引起的血管损伤。
【Objective】To investigate the effects of folic acid on rats＇ atherosclerosis induced by HHcy.【Methods】26 healthy 8-week-old SD male rats,weighing（200±20 g）,after being fed adaptable for a week,were randomly divided into control group（n=6）,model group（n=10）,folic acid prevention group（n=10）.The rats of control group were fed with normal food,and the rats of model group were raised with food including normal food and 2% methionine.The rats of folic acid prevention group were raised with model group diet and 0.0003% folic acid.12 weeks later,the plasma homocysteinemia（Hcy） concentration was detected by high performance liquid chromatography.The lesion of thoracic aorta was observed by hemotoxylin and eosin（HE） staining,oil red O staining and electron microscope.【Results】The model group plasma Hcy levels were significantly higher than the control group（P〈0.01）,folic acid prevention group Hcy levels were significantly lower than the model group（P〈0.01）.Model group can be seen lipid-accumulation and the formation of foam cells through electron microscope,HE and oil red O staining.Folate prevention group did not change significantly to compare with normal group.【Conclusion】HHcy can induce the formation of early atherosclerosis in SD rats,and earlier folic acid interference can effectively prevent the vascular injury by HHcy.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2010年第3期354-357,共4页
China Journal of Modern Medicine
基金
河南省医学科技创新人才工程课题(No:200567)
作者简介
[通信作者]王淑秀，E—mail：wangshuxiu@xxmu．edu．cn
