摘要
本文用１４种识别６碱基的限制性内切酶ＡｐａⅠ、ＢａｍＨⅠ、ＢｇｌⅠ、ＢｇｌⅡ、ＤｒａⅠ、ＥｃｏＲⅠ、ＥｃｏＲⅤ、ＫｐｎⅠ、ＰｖｕⅡ、ＰｓｔⅠ、ＳａｃⅠ、ＳａｌⅠ、ＳｍａⅠ和ＸｈｏⅠ研究了来自我国１２个省和自治区共计１８个地方山羊品种２１８个个体ｍｔＤＮＡ的ＲＦＬＰ，并运用Ｎｅｉ氏公式计算了各限制性类型间的遗传距离Ｐ和群体遗传多态度π值。结果表明，在研究的所有个体中共检测到４１个酶切位点，１８种限制性态型，其中ＢａｍＨⅠ、ＢｇｌⅡ、ＤｒａⅠ、ＥｃｏＲⅠ和ＳａｌⅠ共５种酶表现出多态。１８种限制性态型可归结为６种基因单倍型，单倍型Ⅰ（ＢａｍＨⅠＢ、ＢｇｌⅡＡ、ＤｒａⅠＡ、ＥｃｏＲⅠＡ和ＳａｌⅠＡ）和单倍型Ⅱ（ＢａｍＨⅠＢ、ＢｇｌⅡＡ、ＤｒａⅠＡ、ＥｃｏＲⅠＡ和ＳａｌⅠＢ）为两种基本单倍型，研究结果提示我国地方山羊品种起源于两种不同的母系祖先。各限制性类型间的平均遗传距离为０００４３６，整个群体的平均遗传多态度π值为００４８７％，表明我国地方山羊品种ｍｔＤＮＡ遗传多样性比较贫乏，分化程度较低。
Fourteen restriction endonucleases recognizing 6 base pair,Apa Ⅰ,BamH Ⅰ,Bgl Ⅰ,Bgl Ⅱ,Dra Ⅰ,EcoR Ⅰ,EcoR Ⅴ,Kpn Ⅰ,Pst Ⅰ,Pvu Ⅱ,Sac Ⅰ,Sal I,Sma Ⅰ and Xho Ⅰ were used to investigate the mitochondrial DNA(mtDNA)diversity of 218 individuals of 18 native goat breeds from 12 provinces and autonomous region in China.The genetic distance between haplotypes and the polymorphic degree(π value)were also calculated according to the methods of Neis.The results indicated that 5 restriction endonucleases,BamH Ⅰ,Bgl Ⅱ,Dra Ⅰ,EcoR Ⅰ and Sal Ⅰ,were polymorphic,and 41 restriction sites and 18 restriction morphs which could be sorted into 6 haplotypes were detected.It was shown that haplotype Ⅰ(BamH Ⅰ B,Bgl Ⅱ A,Dra Ⅰ A,EcoR Ⅰ A and Sal Ⅰ A)and Ⅱ(BamH Ⅰ B,Bgl Ⅱ A,Dra Ⅰ A,EcoR Ⅰ A and Sal Ⅰ B)were two main haplotypes.It was inferred that the native goat breeds in China might origin from two different maternal ancestors.The average genetic distance between haplotypes and the polymorphic degree of populations were 0 00436 and 0 0487% respectively.It was also inferred that the genetic diversity and the differentiation of native goat breeds in China was low.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1999年第4期313-319,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家科委基础司专项经费
国家杰出青年基金
