摘要
香荚兰的幼茎在MS＋BA1μg／mL＋NAA5μg／mL培养基上培养40d，其表面形成白色、块状的愈伤组织。愈伤组织在MS＋2，4－D1μg／mL＋BA1μg／mL＋NAA4μg／mL＋2．5％sucrose的半固体培养基上快速增殖培养，培养4周后培养物的重量增加7倍左右；在相同培养基的悬浮培养中，培养4周后培养物的重量增加6～8倍。从不同的培养物中分离出香兰素并测定其含量。
Young stems of vanilla (Vanilla planifolia) were cultured on MS medium containing1μg/mL BA and 5 μg/mL NAA, and white calli produced on the surface in 40 days. Thecalli were then transferred onto half-strength or liquid MS mediums supplemented with1 μ g/mL 2,4-D, 1 μg/mL BA, 4 ppm NAA and 2.5 % sucrose. The cultures resultedweighed around 7-fold on both mediums after 4 weeks. The vanillin from the cultures underdifferent cultural conditions was tested and compared. The results showed that the vanillincontent of the cultures on the medium with 2,4-D was 4-fold higher than that without 2,4-D,and increased by 1/3, about 45 % of that of the mature pods, when the calli was culturedwith 500 μg/mL Of ferulic acid for 1 week.
出处
《热带作物学报》
CSCD
1999年第1期44-48,共5页
Chinese Journal of Tropical Crops
基金
海南省自然科学基金
