摘要
目的研究livin基因短发夹RNA(shRNA)对恶性胶质瘤生长和凋亡的影响。方法将构建的livinβ基因shRNA真核表达载体以脂质体法转染C6细胞。应用Westernblot检测livin表达水平;采用MTT法检测细胞增殖;采用流式细胞术检测细胞凋亡的变化。结果 Livin基因的shRNA可显著抑制C6细胞livin表达(P<0.01),转染组细胞生长受到显著抑(P<0.01),C6细胞凋亡率明显增高,细胞周期出现明显的G2/M阻滞。结论 Livin基因的shRNA导入C6胶质瘤细胞后抑制了livin基因在人胶质瘤细胞C6中的表达,并显著地诱导C6细胞发生凋亡。
Objective To study the effects of livin short hairpin RNA（shRNA） on growth and apoptosis of glioblastoma cell line.Methods The specific shRNA eukaryotic expression vector targeting human livin gene was transduced into C6 glioblastoma cell line by liposome mediated transfection.The expression of livin gene in C6 cell was detected by Western blot.The growth of C6 cell was detected by MTT method.The change of cell apoptosis rate was measured by flow cytometry.Results The expression of the livinβ mRNA was inhibited by livin shRNA.The cell growth in the transfected group was inhibited significantly（P〈0.01）.Glioblastoma cell line C6 suffered a notable apoptosis and G2/M arrest after RNA interference mediated by siRNA targeting livin（P〈0.01）.Conclusion After transduction of the shRNA targeting human livin gene into C6 glioblastoma cell line,the expression of livinβ gene can be inhibited remarkably and the apoptosis of C6 cell can be induced significantly.
出处
《江苏医药》
CAS
CSCD
北大核心
2010年第20期2441-2444,共4页
Jiangsu Medical Journal
