摘要
研究凝结芽孢杆菌(Bacillus coagulans)对尖吻鲈(Lates calcarifer)生长、消化酶及血清非特异性免疫酶活性的影响。把初始菌浓度为10×109cfu/g的凝结芽孢杆菌的粉末制剂分别以0、0.1%、0.5%和1.0%的比例添加到饲料中,投喂初始体重为(18.95±0.27)g的尖吻鲈,尖吻鲈养于0.5m3的玻璃纤维桶中,每桶20尾,每组饲料3个平行,养殖时间50d。尖吻鲈的生长在组间没有显著性差异(P>0.05),但增重率和特定生长率均在0.5%组最大,分别比对照组增加9.54%和4.01%。凝结芽孢杆菌对尖吻鲈的肠和幽门垂消化酶活性没有显著性影响,但胃淀粉酶在1.0%组显著高于对照组,肝蛋白酶在0.5%组显著低于对照组(P<0.05)。血清碱性磷酸酶、过氧化物酶及超氧化物歧化酶在组间均没有显著性差异(P>0.05)。本研究表明,凝结芽孢杆菌对尖吻鲈的生长有一定的促进作用,并可以影响其肝蛋白酶和胃淀粉酶的活性,而对其血清免疫酶的影响很小。
The aim of this research was to evaluate the effects of Bacillus coagulans on growth performance,digestive enzymes and some serum nonspecific immune enzymes in cultured sea bass （Lates calarifer） .Four levels of B.coagulans powder product was supplemented to a basal diet at 0 （the control group） ,0.1 % （group 1） ,0.5% （group 2） and 1.0% （group 3） g/kg feed,respectively.Diets were fed to sea bass with initial body weight of （18.95 ± 0.27） g for 50 days.The sea bass was randomly distributed into 4 groups,each group with 3 replicates in circular fiberglass reinforced plastic tanks with volume of 0.5 m3.The results showed that the weight gain and the specific growth rate were highest in group 2,and were 9.54% and 4.01% higher than that of the control,respectively,but there was no significant difference among the groups （P〉0.05） .There was no significant difference in the digestive enzymes of the intestinal and pyloric caeca within groups.But the liver protease of the group 2 was significantly lower than that of the control,while the amylase of stomach of the group 3 was significantly higher that of the control （P〈0.05） .There was no significant difference in some serum immune enzymes （AKP,POD,SOD） among groups （P〉0.05） .This research indicated that the B.coaglucans could improve the growth of the sea bass slightly.It affected the liver protease and the stomach amylase,too.The serum was slightly affected.
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2010年第6期792-797,共6页
Journal of Shanghai Ocean University
基金
广东省科技计划项目(2005B20301012
2006A20301004)
农业科技成果转化资金项目(2007GB23260379)
关键词
尖吻鲈
凝结芽孢杆菌
消化酶
生长特性
非特异性免疫酶
Lates calarifer
Bacillus coagulans
digestive enzymes
growth performance
nonspecific immune enzyme
作者简介
袁丰华（1983-），女，硕士研究生，专业方向为水产动物营养与免疫。E—mail：yuanwind1983@126．com
通讯作者：李卓佳，E-mail：zhuojiali609@163．com
