摘要
流感疫苗血凝素含量的测量,采用单向放射免疫扩散法,其关键在于扩散环直径的确定。传统采用游标卡尺或高清扫描图像方法测量扩散环直径,存在人为干扰,测量精度与稳定度低。本文基于机器视觉技术研制了血凝素含量自动测量仪,利用CCD相机采集扩散环图像,用图像处理算法测量扩散环直径,实现了扩散环直径数据获取、血凝素含量计算与结果数据分析等操作的自动化与智能化,有效消除人为干扰,提高测量精度。实验表明,该测量仪测量精度可达2μg/mL,相关参数R2>0.99,稳定度高,变异系数小于1%,满足实际测量需求。此仪器适应于各种型别的流感疫苗血凝素含量测量,且测量结果易于保存、传输,便于二次处理。
Using single radial immunodiffusion method to measure the Hemagglutinin Antigen（HA） content of influenza vaccines,the key is to determine the proliferation ring diameters.The traditional methods for the measurement of proliferation ring diameters use a vernier caliper or high-definition scans,which all have human disturbance that result in low measurement accuracy and stability.Based on machine vision technology,automatic measuring instrument for HA content is studied.The getting proliferation ring image by CCD camera and caculating the proliferation ring diameters by image processing,realize the operations automation and intelligence for acquisition of proliferation ring diameters data,calculation of HA content and analysis of results,which effectively improves the measurement accuracy,and eliminates the human disturbance.Experiments show that the measurement accuracy of this instrument is up to 2μg/mL,with relationship coefficient more than 0.99（R20.99）,and high stabilization of less 1% coefficient of variation,that meets the measurement needs.The instrument is suitable for every kinds of HA content.The results are easy to store,transport,and easy to secondary treatment.
出处
《光电工程》
CAS
CSCD
北大核心
2010年第11期78-83,共6页
Opto-Electronic Engineering
基金
吉林省科技基金资助项目
关键词
机器视觉
血凝素含量
单向免疫扩散
扩散环检测
machine vision
HA content
single radial immunodiffusion
detection of proliferation ring
作者简介
作者简介：景文博（1980-），男（汉族），湖北庶城人。讲师，博士生，主要研究工作是图像处理、光电检测。E-mail：wenbojing@sina．com。
