摘要
对９例正常妊娠者和９例重度妊高征者的胎盘组织，用还原型辅酶Ⅱ－黄递酶（ＮＡＤＰＨ－ｄ）组织化学法研究一氧化氮合酶（ＮＯＳ）的组织分布，比色法测定ＮＯＳ活性。结果发现，ＮＯＳ主要分布于合体滋养细胞中。正常组大多数合体滋养细胞中的蓝色颗粒聚集成团块，主要位于细胞的基底部；妊高征组大多数合体滋养细胞中的蓝色颗粒呈弥散状于细胞核周围，无基底部聚集趋势，着色强度明显较正常组弱，两组血管内皮细胞中ＮＯＳ的分布无差异。妊高征组胎盘组织中ＮＯＳ活性显著降低。
By NADPH d histochemical method we studied the distribution of NOS and by colorimetry we determined the activity of NOS in placental tissues from 9 normal pregnant women and 9 severe PIH women The results indicated that the NOS primarily distributed in the syncytiotrophoblast,the blue granules gathered to mass and appeared primarily basal in location in the majority syncytiotrophoblast of normal group In the majority syncytiotrophoblast of PIH group,the blue granules appeared primarily diffuse around nucleus,the staining intension was weaker than normal No difference in the nitric oxide synthase distribution in vessel endothelial cells was found Placental tissues from PIH group had significantly lower activities of NOS than normal group
出处
《山东医药》
CAS
北大核心
1999年第8期1-2,共2页
Shandong Medical Journal
关键词
妊娠高血压
综合征
胎盘
一氧化氮合酶
Pregnancy induced hypertension Nitric oxide synthase Placenta Histochemical method Activity determinrion
