摘要
目的:通过对rhTNF-α生物活性方法的改进,建立了较规范的活性测定方法,避免了人为判定的误差,提高了测定精确性.方法:本研究应用L929鼠结缔组织来源纤维母细胞系,参照国际标准品测定rhTNF-α效价.分别用Northern Dot Blot杂交检测及DNA电泳等方法,观察不同效价rhTNF-α对HL-60人白血病早幼粒细胞生长的影响.结果:rhTNF-α对HL-60细胞的生长抑制作用与时间、剂量正相关,核酸水平检测初步发现c-myc基因表达量降低,且rhTNF-α对DNA有损伤作用.结论:rhT-NF-α的测定可用自动分析的方法进行.rhTNF-α抑制HL-60细胞生长,其机理不同于阿霉素.
Through the improvement on determination of rhTNF-α bioassay, we established the formal procedure . Methods; We choose L929 cell for rhTNF-α bioassay determination. To identify the influence of rhTNF-α on the growth of HL-60 cells, various concentration and effecting time were studied. Results: The results showed that the inhibition of rhTNF-α on HL-60 cells based on dose and time. Northern Dot Blot and DNA electrophoresis showed c-myc gene was surpressed, and DNA was damaged by rhTNF-α. Conclusion: Autometic calculation is responsible for bioassay of rhTNF-α. rhTNF-α could inhibit the proliferation of HL-60 cells and its mechanism is different from doxirubicin.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
1999年第2期141-144,共4页
Chinese Journal of Cancer Biotherapy
