摘要
报告一种大鼠活体多次采血法。用１ ％ 戊巴比妥钠腹腔麻醉后，剑突下进针，斜行向上刺入２－５ ～３ ．０ｃｍ 即达心脏，回抽。３０ 只ＳＤ大鼠，间隔１０ ｄ 采血２ ｍｌ，共４ 次，测定Ｈｂ、ＲＢＣ、Ｈｃｔ、ＷＢＣ、ＡＬＴ、ＴＰ、ＢＵＮ。结果：采血１ ０００ 余次，１ 次穿刺成功率９８ ％ 以上，且大鼠成活，各次血液成份及生化指标差异均无显著性（ Ｐ＞ ０－０５） 。本法操作简便，定位准确，采血量大，可重复采血。
We have established a new method which can gather more blood repeatedly in living rats.The rats were anaesthetized by intraperitoneal injection with 1% sodium pentobarbital,then penetrated upward obliquely at the site of just below the xiphoid,entered about 2.5～3.0 cm into the heart and drew blood out.Two ml blood has been extracted from 30 SD rats every 10 days 4 times in all,and the Hb,RBC,Hct,WBC,ALT,TP and BUN have been determined.Results:by using this method,the rate of one time successful penetration is over 98% without sacrificing animals,and no significant difference ( P >0.05) has been detected in blood components and biochemical targets each time.The method is easy to control and is applicable for gathering more blood repeatedly in living rats.
