摘要
目的探讨黄芪总皂苷(AST)与黄芪甲苷(AST-Ⅳ)在人工胃液(0.1mol·L-1HCL溶液)及氨试液中的含量变化情况。方法采用紫外分光光度比色法测定黄芪总皂苷含量,检测波长:531nm;采用HPLC-ELSD法测定黄芪甲苷含量,以Eclipse XDB(150mm×4.6mm,5μm)ODS色谱柱,流动相为乙腈:水(梯度洗脱),洗脱梯度为0-17min,乙腈26%→55%,流速为1.0mL·min-1,检测器为ELSD-Alltech3300,漂移管温度为40℃,氮气流速为1.5L·min-1。结果单纯的黄芪甲苷在0.1mol·L-1HCL溶液中降解14%左右,再经4mL8%氨试液处理后含量变化不大;黄芪总皂苷在0.1mol·L-1HCL溶液中降解了60%左右,总皂苷中的甲苷降解了58%左右;将总苷先酸解再经4mL8%氨试液处理,则其中的黄芪甲苷含量降低约23%。结论黄芪总皂苷及黄芪甲苷在0.1mol·L-1HCL溶液中含量均下降,先酸解再经4mL8%氨试液处理后总苷中的黄芪甲苷含量虽有所提高,但仍比原含量低。
Objective To observe the changes of astragalosides（AST）and astragalosideⅣ（AST-Ⅳ）in 0.1 mol·L-1 HCL solution（artificial gastric juice） and in the ammonia.Methods The total content of AST was determined by ultraviolet spectrocolorimetric analysis with detection wavelength of 531 nm.The content of AST-Ⅳ was determined by HPLC-ELSD on Eclipse ODS column（150×4.6 mm,5 μm）with acetonitrile（26 %→55 %）-water（gradient elution for 0-17 min）as the mobile phase,the flow rate was 1.0 mL·min-1,the temperature of drift tube was 40 ℃,and the flow rate of nitrogen gas（N2）was 1.5 L·min-1.Results Purified AST-Ⅳ in 0.1 mol·L-1 HCL solution was degraded about by 14 %,but remained unchanged in 8 % ammonia.In 0.1 mol·L-1 HCL solution the total of AST was degraded about by 60 % while the content of AST-Ⅳ in AST was degraded about by 58 %.When AST was hydrolyzed first in 0.1 mol·L-1 HCL solution and then in the 4 mL 8 % ammonia,the content of AST-Ⅳ in AST was degraded about by 23 %.Conclusion The content of both AST and AST-Ⅳ decreases in 0.1 mol·L-1 HCL solution.Although the content of AST-Ⅳ in AST is increased by hydrolyzing first in 0.1 mol·L-1 HCL solution and then in the 4 mL 8 % ammonia,it is still less than the initial.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2010年第5期533-537,共5页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
教育部科学技术研究重点项目(208104)
广东省科技计划项目(2007B031404011)
作者简介
作者简介：冯小权（1986-），女．硕士研究生，研究方向：中药新药研究与指纹图谱分析。
通讯作者：黄可儿，研究员，博士生导师，主要从事中西医结合临床及药理研究。Email：hke8989@163.com.
