摘要
目的:检测白细胞介素-18(IL-18)在胎膜早破(PROM)患者胎膜和血清中的表达,探讨IL-18与发生PROM的关系,分析IL-18作为诊断PROM生物学指标的可能性。方法:用酶联免疫吸附试验(ELISA)和免疫组化SP法分别检测PROM患者42例(PROM组)和正常孕妇28例(正常妊娠组)血清和胎膜中IL-18的表达。结果:PROM组血清中IL-18水平和胎膜IL-18表达强度均较正常妊娠组显著升高(P(0.01),但随破膜时间的延长差异无统计学意义(P>0.05);PROM组中合并亚临床绒毛膜羊膜炎者血清IL-18水平和胎膜IL-18的表达强度均较非绒毛膜羊膜炎者显著升高(P(0.01);血清和胎膜IL-18含量呈正相关(P(0.05)。结论:PROM组血清和胎膜组织中IL-18水平明显高于正常妊娠组,这一趋势在破膜早期即明显表现出来,且与破膜时间无关。因而IL-18有可能作为1个对PROM的预测指标。
Objective：To detect the expression and distribution of interleukin-18 （IL-18） in premature rupture of membrane （PROM） as well as its clinicalpathological significance.Methods：Twenty-eight normal term-pregnant women as control, and forty-two women with PROM were enrolled. IL-18 levels in maternal sera and fetal membranes were measured by enzyme-linked immunosorbent assay （ELISA） and immunohistochemistry. Sub-clinical chorioamnionitis was diagnosed histologically.Results：The expression of IL-18 in maternal serum and fetal membranes of PROM was significantly higher than those in normal pregnancy group（P〈0.05）, and they did not increase in a PROM time-dependent manner （P〉0.05）.The same results also occurred in PROM with and without sub-clinical chorioamnionitis （P〈0.05）.There was a positive correlation between IL-18 levels in maternal sera and protein expressions in fetal membranes（P〈0.05）.Conclusion：IL-18 highly expresses in PROM and this trend occurrs in the early period of PROM. There is possible of IL-18 to be a predictable index of PROM.
出处
《中国妇幼保健》
CAS
北大核心
2010年第27期3957-3960,共4页
Maternal and Child Health Care of China
