摘要
临床获取的人体肾结石固体样品难以在表面解吸常压化学电离质谱(DAPCI-MS)中获得满意的信号,将其制备成乙酸-甲醇溶液后直接滴加在信封纸表面,在无需脱盐处理的条件下,可以采用DAPCI-MS直接分析。结果表明:DAPCI-MS能够承受结石样品中的大量基体,快速测定结石中的三聚氰胺和尿酸等成分,并鉴定其结构。本方法操作简单方便,准确度高,为三聚氰胺引起的肾结石的临床诊断提供了科学依据。
The clinical human kidney stone samples that generated no satisfactory signal in direct analysis using surface desorption atmospheric pressure chemical ionization mass spectrometry（DAPCI-MS） were dissolved in acetic acid/methanol solutions,and then deposited on a paper surface for DAPCI-MS analysis without desalination.The results showed that the melamine and the uric acid as well,in the stone samples were rapidly detected and identified by using DAPCI-MS2.The detection limits were about 3.4×10-15 g/mm2 for melamine on the paper surface.The analytical results obtained using DAPCI-MS were validated by using conventional ESI-MS.The data demonstrate that DAPCI-MS2 is a promising technique for clinical diagnosis of nephrolithiasis caused by either melamine or uric acid,particularly because of its high specificity,high throughput and good accuracy.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2010年第9期1325-1328,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金仪器专项(No20827007)
国家科技部创新方法专项基金(No2008IM040400)资助项目
关键词
表面解吸常压化学电离源
质谱
肾结石
三聚氰胺
尿酸
Surface desorption atmospheric pressure chemical ionization
Mass spectrometry
Kidney stone
Melamine
Uric acid
作者简介
E-mail：chw8868@gmail．comzxtjxgz888@sohu．com
