摘要
以姜荷花新品种"红观音"球茎为外植体进行组织培养与快速繁殖研究。结果表明:外植体经灭菌处理后接种到MS+BA2～3mg/L+NAA0.2～0.3mg/L培养基上,均能较好地诱导休眠芽的生长;丛生芽在18号培养基(花宝2号1.5g/L+MgSO40.15g/L附加MS铁盐及其维生素和其它有机物+椰子汁100mL/L)+TDZ0.5mg/L培养基上增殖效果最佳,增殖系数可达3.73,且玻璃芽少;生根培养基以18号培养基+NAA0.5mg/L效果最好,生根率为92%;试管苗在以园土:泥炭土:珍珠岩=1:1:1(V/V/V)的栽培基质中,成活率可达95%以上。
Tissue culture and rapid propagation of Curcuma alismatifoliacv.Red Guanyin were studied.When sterilized corms that were cut into piece with 1-2dormancy buds were cultured on MS medium with 2-3mg/L 6-BA and 0.2-0.3mg/L NAA,budding and cluster buds could be induced.The proliferation rates were 3.73in H18（Haponex 1.5g/L and MgSO40.15g/L instead of macro-element and micro-element of MS）＋coconut milk 100mL/L＋TDZ 0.5mg/L.The best medium for rooting was H18contained 0.5mg/L NAA and the rooting rate was 91.43%.When the test-tube plantlets were transplanted on humus：sphagnum：perlite=1：1：1（V/V/V）,their survival rate of was over 95%.
出处
《北方园艺》
CAS
北大核心
2010年第18期154-156,共3页
Northern Horticulture
基金
广州市重大科技攻关计划资助项目(2004Z1-E0041)
关键词
姜荷花
观赏植物
组织培养
快速繁殖
Curcuma alismatifolia
ornamental plants
tissue culture
rapid propagation
作者简介
商宏莉（1965-），女，硕士，副教授，现从事遗传育种和生物技术研究工作。E-mail：honglishang0507@163．com。
通讯作者：曾宋君（1965-），男，副研究员，现主要从事植物遗传育种和生物技术研究工作。E-mail：zengsongjun@scib．ac．cn。
