摘要
利用扩增片段长度多态性技术(AFLP)分析了13个条斑紫菜品系,在80对引物中,有11对能得到重复性好的多态性扩增条带,共扩增出619条谱带,每对引物扩增带数为40—77条,并且各对引物扩增结果均显示样品间存在差异。共得到609条多态性条带,多态位点的比例高达98.38%。根据AFLP图谱计算了不同样品间的遗传距离(GD)和相似性系数(GS)。聚类结果显示,品系海安与二室间的遗传相似系数较高而聚合在一起,不同样品间的相似性系数介于0.595—0.845之间。结果表明,条斑紫菜的遗传多样性极为丰富,而部分材料间的遗传距离有随地理间距增大而逐渐增大的趋势。聚类分析结果反映了目前条斑紫菜养殖品种间存在着相互混杂。
Amplified fragment length polymorphic technique was used to analyze the genomic DNA polymorphism of 13 lines of Porphyra yezoensis.Eleven of the 80 AFLP primer pairs gave reproducible polymorphic DNA amplification patterns,and 619 bands were obtained.The amplified bands ranged from 40 to 77 per primer pair showed the genetic diversity among these samples.Six hundred and nine of AFLP bands were polymorphic loci.The proportion of polymorphic loci was 98.38%.The genetic distance（GD） and similarity coefficients（GS） were calculated based on the polymorphic data.UPGMA cluster indicated that samples from Haian and Ershi were of high similarity.The GS between these samples was 0.595—0.845.The result shows that the genetic diversity level in P.yezoensis is fairly high.Similar increasing trend was found in genetic distance coefficient and geographical distance.UPGMA cluster indicates that P.yezoensis in our country is miscellaneous.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2010年第4期489-494,共6页
Oceanologia Et Limnologia Sinica
基金
国家高技术研究发展计划(863计划)资助项目
2006AA10A413号
国家科技支撑计划项目
2006BAD09A04-08号
国家自然科学基金重点项目资助
30830015号
作者简介
袁昭岚，2005．四种紫菜自由丝状体遗传多样性的同工酶和ISSR研究．苏州：苏州大学硕士学位论文
通讯作者：王广策，研究员，E—mail：gcwang@qdio．ac．cn
