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基因打靶技术的研究进展 被引量：6

The Research Progress of Gene Targeting
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摘要 基因打靶技术是20世纪80年代发展起来的新技术,是一种利用DNA同源重组原理和胚胎干细胞(ES细胞)技术按定向组合的方式改变生物活体遗传信息的试验手段,具有广阔的应用前景。对基因打靶技术原理、步骤、条件性基因打靶以及应用进行综述。 Gene targeting is a new technique emerged from the 1980s,as an experimental method based on DNA homogenous recombination theory and embryonic stem cell（ES cell） system to modify specific genes and make them express in an organism.It has been applied in some aspects and will be used widely in the future.In this paper,the theory,step,application of gene targeting and conditional genet targeting were reviewed.
作者 张丽娜 刘国安 杨红
机构地区 西北师范大学生命科学学院
出处 《生物技术通报》 CAS CSCD 北大核心 2010年第9期51-55,共5页 Biotechnology Bulletin
基金 省教育厅研究生导师项目(0901-05)
关键词 基因打靶 DNA同源重组 胚胎干细胞 Gene targeting DNA homogenous recombination Embryonic stem cell
分类号 Q78 [生物学—分子生物学]
作者简介 作者简介：张丽娜，女，讲师，硕士研究生，研究方向：生化与分子生物学；E-mail：lina791001@163．com
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参考文献32

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二级参考文献87

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共引文献24

同被引文献84

  • 1刘旭霞,田庚,陈晶.我国转基因生物环境安全监管法律制度研究综述[J].昆明理工大学学报（社会科学版）,2008,8(8):10-15. 被引量：2
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引证文献6

二级引证文献24

生物技术通报
2010年 第9期

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