摘要
研究3个STR基因座（D21S11、HumFGA、D19S253）在广西壮族人群中的基因频率分布及其在实际检案中的应用价值。以自制等位基因Ladder样品作为标准对照，用PCR结合PAGE技术对3个STR基因座的扩增产物进行分型。结果显示：D21S11基因座有14个等位基因，有44个基因型；HumFGA基因座有15个等位基因，40个基因型；D195253基因座有9个等位基因，23个基因型。经检验，3个STR基因座基因型分布均符合Hardy－Weinberg平衡，累计个体识别力（DP）为0．9995。3个STR基因座在壮族人群属高识别力遗传标记系统，在法医学个体识别及亲权鉴定方面有重要价值。
Allele frequency distributions of three STR loci (D21S11' HumFGA.Dl9S253) in Chinese Zhuang ethnic group were studied- The PCR products were analyzedby PAGE, followed by silver staining. Self-made allelic ladder was used as standard sizemarkers. Fourteen alleles and forty-four genotypes of D2lSl1 locus, fifteen alleles and fortygentypes of HumFGA locus, and nine alleles and twenty-three genotypes of D19S253 locuswere observed. The total discrimination P0wer of three loci was O. 9995. Our results showthat these three STR loci can be used for individual identification and paternity testingZhuang ethnic group.
出处
《中国法医学杂志》
CSCD
1999年第1期15-15,8,共2页
Chinese Journal of Forensic Medicine
基金
广西壮族自治区重点科技攻关项目!9817103
关键词
STR基因座
D21S11基因座
基因频率分布
法医学
Short tandem repeate (STR) locus D2lS1l lcous HumFGA locus D19S253 locus Allele frequency distribution Individual identification
