摘要
目的:建立支气管哮喘伴发抑郁的动物模型,探讨支气管哮喘伴发抑郁时机体免疫功能的变化。方法:选择Wistar大鼠,随机分为对照组、哮喘组、哮喘伴发抑郁组,采用经典的卵蛋白(ovalbumin,OVA)激发法略加改进建立哮喘模型,在此基础上给予慢性轻度不可预见性应激刺激(chronic unpredictable mild stress,CUMS)28d复制抑郁模型,综合评价动物模型是否成功,酶联免疫技术测定各组大鼠血液及肺部组织IL-1β、IL-6的含量。结果:支气管哮喘伴发抑郁大鼠模型建立成功;哮喘伴发抑郁组大鼠血液及肺部组织IL-1β含量,与对照组比较明显升高(P<0.05),与哮喘组比较亦有增高趋势;哮喘伴发抑郁组大鼠血液及肺部组织IL-6含量,与对照组比较明显升高(P<0.05),与哮喘组比较亦有增高趋势。结论:OVA激发加CUMS可成功制备支气管哮喘伴发抑郁大鼠模型,该模型血液及肺部组织IL-1β、IL-6的含量明显升高。
Objective：To establish the animal model of bronchial asthma combined with depression and explore its changes of the levels of IL-1β and IL-6.Methods：Tirty-two wistar rats were randomly divided into control group,bronchial asthma group and bronchial asthma combined with depression group.The latter two groups were sensitized with ovalbumin for 28 days and the bronchial asthma combined with depression group was followed by CUMS for 28 days.Levels of IL-1β and IL-6 in serum and in lung tissue were measured by ELISA.Results：Bronchial asthma combined with depression animal models were successfully established.The levels of IL-1β and IL-6 in serum and in lung tissue of the bronchial asthma combined with depression group were all significantly increased compared with the control group（P 0.05）,and had an increased trend compared with the bronchial asthma group.Conclusion：The bronchial asthma combined with depression animal model can be established by sensitizing with ovalbumin and followed by CUMS,and its levels of IL-1β and IL-6 in serum and in lung tissue increased.
出处
《中国临床心理学杂志》
CSSCI
CSCD
2010年第4期418-421,共4页
Chinese Journal of Clinical Psychology
基金
山东省教育厅科技计划项目(J07YE03)
山东省自然科学基金资助项目(ZR2009CL045)
教育部人文社会研究基金项目(07JAXLX005)
作者简介
通讯作者：孙宏伟
