摘要
目的研究ｎｍ２３－Ｈ１基因在口腔鳞癌中的蛋白表达，试探讨其与口腔鳞癌颈淋巴结转移的关系及意义。方法采用免疫组化ＳＡＢＣ法检测７５例口腔鳞癌中ｎｍ２３－Ｈ１蛋白染色，利用卡方检验及多因素Ｌｏｇｉｓｔｉｃ逐步回归进行统计分析。结果ｎｍ２３－Ｈ１蛋白染色阳性信号主要定位于肿瘤和上皮细胞胞浆中。淋巴结转移组ｎｍ２３－Ｈ１，蛋白高染率为１５．１５％（５／３３），无淋巴结转移组高染率为５２．３８％（２２／４２），两组间有极显著差异（Ｐ＜０．０１）。ｎｍ２３－Ｈ１蛋白染色水平与病理分级有关（Ｐ＜０．０５），同肿瘤大小、部位、患者的年龄、性别无关。多因素分析发现颈淋巴结转移与ｎｍ２３－Ｈ１蛋白表达水平关系最密切（ＯＲ＝０．１８４６）。结论ｎｍ２３－Ｈ１基因的蛋白表达水平高低可能是影响口腔鳞癌颈淋巴结转移的重要因素，检测ｎｍ２３－Ｈ１蛋白的表达水平可能对临床判断和预测口腔鳞癌的转移有一定意义。
Objective To
make an inquiry into the relation between the expression level of nm23 H 1 protein and
metastasis of oral squamous cell carcinoma (OSCC).Methods We evaluated the expression of
nm23 H 1 protein in 75 cases of OSCC by immunohistochemical technique (SABC).Results
These immunoreactivities of nm23 H 1 protein were most common in the cytoplasm of tumor
and epithelia cell. Using single factor chi test,we found there was a significant correlation
between the expression of nm23 H 1 protein and lymph node metastasis of OSCC ( P <0 01),
pathology grade ( P <0 05).But no statistical association was found among nm23 H 1 protein
expression level and tumor size, site and patient′s sex, age. Using multi factor logistic
stepwise regression analysis, we found that nm23 H 1 protein expression level had more
effect on OSCC cervical lymph node metastasis than any other parameters.Conclusions The
results suggested that nm23 H 1 protein play an important role in the suppression of the
metastasis of OSCC, and that determining the expression level of nm23 H 1 protein may help
us prognose the metastasis of OSCC.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1999年第1期19-24,共6页
Acta Academiae Medicinae Sinicae
关键词
口腔肿瘤
鳞癌
NM23基因
肿瘤转移
neoplasm metastasis
gene suppressor,
tumor
immunohistochemistry
nm23 gene
