摘要
目的:研究芹菜素(apigenin,AP)对3T3-L1前脂肪细胞增殖及分化的影响。方法:采用3T3-L1前脂肪细胞,利用MTT比色分析法检测不同质量浓度芹菜素对3T3-L1前脂肪细胞增殖的影响;采用传统的鸡尾酒诱导剂诱导分化3T3-L1前脂肪细胞,利用油红O染色检测,通过比色分析法检测不同质量浓度芹菜素对3T3-L1前脂肪细胞分化的影响。结果:30、50μg/mL芹菜素相对于空白组和阳性对照组能够显著抑制3T3-L1前脂肪细胞增殖和分化,提示其可能具有预防肥胖的作用。
Objective：To investigate the effect of apigenin on proliferation and differentiation of 3T3-L1 preadipocytes.Methods：3T3-L1 preadipocytes were used as target cells to explore the effect of apigenin on their proliferation measured by MTT assay.Besides,cell differentiation using differentiation cocktail,oil red O staining and spectrometric determination were carried out for investigating the effect of apigenin on the differentiation of 3T3-L1 preadipocytes.Results：Apigenin at 30μg/mL and 50 μg/mL concentrations could inhibit the proliferation and differentiation of 3T3-L1 preadipocytes in comparison with the blank group and the positive control group（established using Dulbecco smodified Eagle smedium containing 0.5 mmol/L IBXM,1μmol/L dexamethasone and 10μmol/L insulin）.Conclusion：Apigenin appear to have potential for the prevention of obesity.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第15期260-263,共4页
Food Science
关键词
芹菜素
3T3-L1
增殖
分化
肥胖
apigenin
3T3-L1 preadipocytes
proliferation
differentiation
obesity
作者简介
于华强（1982-），男，硕士研究生，研究方向为食品科学。E—mail：yhqiang2012@yahoo．com．cn
通信作者：籍保平（1958-），男，教授，硕士，研究方向为果蔬加工与功能食品。E-mail：jbping@cau．edu．cn
