摘要
目的：研究可溶性人类白细胞抗原G（sHLA-G）在孕晚期重度子痫前期患者血浆的含量水平及其亚型mRNA在胎盘组织中的表达，探讨sHLA-G的来源及其在子痫前期发病中的意义。方法：选择2008年10月至2009年4月第四军医大学唐都医院重度子痫前期患者20例（重度子痫前期组），20例正常足月妊娠者（正常足月妊娠组）及20例正常未妊娠者（正常未妊娠组），采用酶联免疫吸附法（ELISA）检测血浆sHLA—G的含量；采用实时荧光定量PCR法比较sHLA-G亚型（HLA．G5、G6）mRNA在重度子痫前期组和正常足月妊娠组胎盘组织表达的差异。结果：①重度子痫前期组血浆sHLA—G含量水平明显低于正常足月妊娠组（P〈0．05），正常未妊娠组与重度子痫前期组和正常足月妊娠组比较，差异有统计学意义（P〈0．05）。②重度子痫前期组胎盘HLA．G5、G6mRNA相对表达量（0．32）明显低于正常足月妊娠组相对表达量（1．0）。结论：重度子痫前期患者血浆sHLA-G及其胎盘组织中亚型mRNA含量水平均降低，可能与子痫前期发病有关，胎盘可能是sHLA-G的来源之一。
Objective：To detect the level of sHLA-G protein in plasma and the expression of its isoforms mRNA in placenta in patients with severe preeclampsia during the third trimester, to explore the origin of sHLA-G and its significance in preeclampsia. Methods：①The level of sHLA-G in plasma was detected by Enzyme linked immunosorbent assay （ELISA） in severe preeclampsia group （n =20）, normal pregnancy group （n=20） and unpregnancy group （n=20） from October, 2008 to April, 2009 in Tangdu hospital;the expression of isoforms mRNA of sHLA-G（ HLA-G5 ,G6）in placenta in severe preeclampsia group and normal pregnancy group was checked by Real - time fluorescence quantitative PCR （RT-PCR） . Results：①The plasma level of sHLA-G in severe preeclampsia group was much lower than that in normal pregnancy group （P 〈 0, 05）. Matemal plasma HLA-G levels in unpregnancy group were significantly different from those in normal pregnancy and preeclampsia group （P 〈0.05）. ②The relative expressions of HLA-G5, G6 mRNA in preeclampsia group（0.32） were obviously lower than those in normal pregnancy .group（1.0）. Conclusions： A decrease of sHLA-G protein in plasma and the low expression of sHLA-G isoform mRNA in placenta of pa- tients with severe preeclampsia may associate with the pathogenesis of preeclampsia, and placenta could be one of the origins of sHLA-G.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2010年第7期525-528,共4页
Journal of Practical Obstetrics and Gynecology
基金
973项目(编号:2007CB948102)
作者简介
通讯作者：姚元庆，Email：yqyao@hotmail．com
