摘要
目的探讨可溶性人类白细胞抗原Ⅰ（ｓＨＬＡⅠ）定量检测在监控和预测器官移植排斥反应中的作用。方法采用酶联免疫吸附试验（ＥＬＩＳＡ）双抗夹心法，以Ｗ６／３２单克隆抗体包被酶标板，加被检血清，再加抗β２ｍ辣根过氧化物酶及底物显色。以不同浓度ｓＨＬＡⅠ标准品的结果绘出标准曲线。测定６５名正常人ｓＨＬＡⅠ含量，并对２例异基因骨髓移植和１０例肾移植患者术后连续检测ｓＨＬＡⅠ。结果本组正常人样本血清中ｓＨＬＡⅠ含量为（８０７１２±５１９１８）μｇ／Ｌ；异基因骨髓移植和肾移植患者术后无排斥反应者ｓＨＬＡⅠ水平无明显变化；发生排斥反应者则有不同程度升高。结论ｓＨＬＡⅠ的检测可能用于监控移植后排斥反应。
Objective To introduce the method of quantification of sHLA Ⅰ antigens. Method Sandwich ELISA assay was used. After microtiter plate coated by W6/32 mAb, serum was added, followed by incubation with anti β 2m HRP and the color development by substrate was added.Results Standard curve was obtained by quantification of sHLA Ⅰ standard reagent in series dilution. The level of sHLA Ⅰ antigens in normal individual was (807.13±519.18) ng/ml Samples of 2 allogeneic bone marrow transplantation patients and 10 renal transplantation patients were serial tested by this method.Conclusion sHLA Ⅰ level shows no marked variations in patients without rejective reaction and increases in vary in a level in patients with rejective episods.
