摘要
目的 观察SEPS1基因在内毒素所致脓毒症小鼠肝脏的变化规律.方法 采用内毒素攻击小鼠脓毒症模型.BALB/c小鼠84只,随机选取对照组10只,其余为脓毒症组.对照组麻醉后活杀动物.脓毒症组腹腔注射内毒素10 mg/kg后,分别于6、12、24、48、72、96 h留取肝脏和血标本,每个时间点随机选取10只小鼠.检测指标包括血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）和乳酸脱氢酶（LDH）;肝组织内肿瘤坏死因子（TNF-α）和白细胞介素6（IL-6）含量;肝组织SEPS1蛋白表达采用Western印迹检测.结果 与正常对照组比较,脓毒症组肝脏有损伤,表现为ALT、AST和LDH含量升高,24 h达到高峰,分别为（99±11）、（299±48）和（1523±131）U/L（与0 h比较,均P〈0.05）.随后逐渐下降,并于24～72 h恢复至损伤前水平.IL-6和TNF-α水平明显升高,0-48 h内损伤持续快速加重,IL-6和TNF-α均在48h达到高峰,分别为（30 325±17 901）ng/L和（36 509±20 794）ng/L[与0 h（23 802±11 150）、（29 799±8239）ng/L比较,均P〈0.05].Western 印迹检测显示脓毒症组小鼠肝脏SEPSl蛋白表达显著升高,并于伤后24 h达峰值.随后逐渐减弱,72 h近乎恢复到正常水平.免疫组化结果显示脓毒症组小鼠肝脏SEPS1蛋白表达增高,并于伤后第24小时达峰值.病理结果显示脓毒症小鼠的肝组织明显病变,6-12h细胞病变最严重.结论 内毒素所致脓毒症小鼠肝脏有不同程度的损伤,IL-6和TNF-水平明显升高.肝脏SEPS1蛋白表达显著升高,并于伤后24 h达峰值,72 h恢复到接近正常水平.
Objective To investigate the change of selenoprotein S1 （ SEPS1） hepatic expression in septic mouse induced by LPS attack. Methods The septic murine model induced by LPS attack was established. Ten mice were randomly selected as control group from 84 BALB/c mice and others as septic group. The mice were sacrificed after anesthesia in control group and 10 mg/kg LPS was injected intraperitoneally into septic group mice. Liver and blood samples were taken at 6, 12, 24, 48, 72 and 96 h after LPS injection. Ten mice were randomly selected at each time point The levels of blood ALT, AST,LDH and liver IL-6, TNF-a were detected. And the SEPS1 expression was simultaneously detected by Western blot Results There was liver damage in septic group compared with normal control group. The levels of ALT, AST and LDH markedly increased And all peaked at 24 h. The levels were （99±11 ） ,（299±48） and （1523±131） U/L respectively （ versus level at zero hour, P 〈 0. 05）. Then there was a gradual decrease and the pre-damage levels were restored during 24-72 h. The levels of IL-6 and TNF-a also markedly increased. The damage deteriorated rapidly and peaked at 48 h. The levels were （30 325±17 901） and （36 509±0 794） ng/L respectively [versus level at zero hour （23 802±11 150）, （29 799±239） ng/L, P 〈0. 05]. Western blot showed that SEPS1 protein expression markedly increased simultaneously in liver of septic mouse. And the peak value was reached at 24 h post-injury. Then there was a gradual decrease and normal level returned at 72 h. Immunohistochemical results showed that SEPS1 protein expression in liver of septic mouse also markedly increased. And the peak value was reached at 24 h postinjury. Pathologic results showed that liver lesion was apparent in septic mouse and it was the worst during 6-12 h. Conclusion Liver damage to different extents may be induced by LPS attack in septic mouse. The levels of IL-6 and TNF-a markedly increase. The SEPS1 protein expression in liver of septic mouse is also markedly elevated. And it peaks at 24 h post-injury and returns to normal at 72 h.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2010年第26期1841-1844,共4页
National Medical Journal of China
基金
国家自然科学基金（30371390）
作者简介
通信作者：何蕾，Email：sms301sms@126．com.
