摘要
目的通过检测结直肠癌组织及粪便中Vimentin基因甲基化探讨其在结直肠癌早期诊断中的应用。方法分别收集结直肠正常新鲜组织、癌新鲜组织及粪便标本,提取组织及粪便基因组DNA,DNA经亚硫酸盐处理后进行甲基化特异性PCR检测,检测结果经3%琼脂糖电泳鉴定。结果正常结直肠组织中未检测到Vimentin基因甲基化,结直肠癌组织中Vimentin基因甲基化的检出率为80%,结直肠癌患者粪便中Vimentin基因甲基化检出率为36%,其中早期直肠癌粪便的Vimentin基因甲基化检出率为66.7%。结论Vimentin基因在直肠癌早期诊断中具有重要意义。
Objective To explore the potential application of Vimentin gene methylation in early clinical diagnosis of colorectal cancer from patients＇ fecal DNA.Methods Genomic DNA were extracted from fresh normal and cancer tissues as well as tumor patients＇ stool.After bisulfite conversion of DNA,the Vimentin gene methylation was detected by methylation-specific PCR and then identified by agrose gel electrophoresis.Results Vimentin gene methylation was not detected in normal colon and rectum tissues while the detecting rate of Vimentin gene methylation was 83% in cancer tissues.In fecal DNA, the detecting rate of methylation was 36% in all colorectal cancer patients and increases to 66.7% if only observing early rectal cancer.Conclusion Vimentin gene methylation has a clinical significance in rectal cancer early diagnosis.
出处
《胃肠病学和肝病学杂志》
CAS
2010年第7期601-603,共3页
Chinese Journal of Gastroenterology and Hepatology
作者简介
通讯作者：盛剑秋，E—mail：jianqiu@263．net
