摘要
首次从中国明对虾（Fenneropenaeus chinensiss）肌肉组织中克隆得到TOR基因的cDNA，全长共7638bp，开放阅读框7389bp，编码2462个氨基酸。氨基酸序列比对和结构域分析均证实其为目前已知的TOR激酶的同源蛋白。应用实时定量PCR的方法研究TOR在中国明对虾中的组织分布特异性，以及注射等浓度的亮氨酸和精氨酸后，肌肉组织中TORmRNA表达水平在3，6，12，24h的变化，探讨营养水平对TOR基因表达的影响。结果显示，注射亮氨酸和精氨酸24h的对虾TORmRNA表达量是对照组的3～4倍，显著升高（P〈0．05），证实氨基酸对TOR的表达具有调节作用。
The full length cDNA of TOR （Target of Rapamycin） gene from the muscle ofFenneropenaeus chinensis was cloned for the first time. The cDNA was 7638 bp, containing the 7389 bp open reading frame, which encoded 2462 amino acids. The deduced TOR amino acid sequences of F. chinensis was compared with other known sequences and domains. The results confirmed their homology. Real-time PCR was used to detect the tissue-specific expression of TOR in F.chinensis and response of TOR mRNA in muscle at 3, 6, 12, 24h to injection of leucine or arginine. The results showed that TOR mRNA was 3-4 times that of the control 24 hours after the injection of leucine or arginine, indicating amino acids had a regulatory role in the expression of TOR.
出处
《海洋科学》
CAS
CSCD
北大核心
2010年第6期71-80,共10页
Marine Sciences
基金
国家863计划资助项目(2008AA100805)
作者简介
作者简介：孙妹娟（1984-），女，硕士研究生，主要从事水产动物分子营养学研究，电话：0532-82898723，E-mail：qiqi1709@163．com；
王雷，通信作者，电话：0532-82898722，E-mail：wanglei@ms．qdioac．cn
