摘要
目的 观察光动力学疗法（PDT）对人胶质瘤细胞株U87-MG抗原加工相关转运体1（TAP1）表达的影响.方法 CCK-8法确立光敏剂最适浓度.实验随机分为5组：A、B、C、D组细胞与光敏剂避光孵育2h后,接受激光照射,能量密度分别0、1.2,2.4A.2J/cm2；E组未予处理（无光敏剂、无激光）.PDT后2、12 h分别收集细胞,实时定量聚合酶链反应（realtime-PCR）检测；12、24 h提取蛋白,进行Western blot分析.结果 处理后TAP1基因转录水平明显升高：2h时,1.2、2.4A.2 J/cm2组分别上调11.070、5.925、4.175倍；12h时,分别上调10.400、4.560、4.023倍.TAP1蛋白在PDT后12 h,0、1.2、2.4、4.2 J/cm2组分别上调1.17、2.65、1.92、1.37倍；24 h,分别上调1.13、2.43、2.38、2.09倍,差异有统计学意义（P〈0.01）.结论 PDT上调TAP1基因的转录和表达,并呈剂量依赖性和时间依赖性,从而增强肿瘤细胞抗原处理及提呈能力.
Objective To investigate the effect of hematoporphyrin monomethyl ether （HMME）-based photodynamic treatment on gene expression of transporter associated with antigen processing 1 （TAP1） in human U87-MG glioma. Methods Cells were incubated with 10 g/L HMME for 2 h and irradiated with fluence of 0, 1.2, 2.4, 4. 2 J/cm2. RNA was isolated at 2 and 12 h post-treatment and from one control （untreated） , and the expression changes were observed by real-time reverse transcription-poly-merase chain reaction （ RT-PCR）. Protein was isolated at 12 and 24 h post-treatment and from one control, and the expression level was detected by Western blotting. Results Post-treatment, the expression of TAP1 mRNA and protein was obviously increased as compared with the control group （all P〈0.01）. Conclusion The therapeutics of HMME-mediated PDT might have more pronounced effect on TAP1 mRNA and protein expression, indicating that the effect may be involved in the antigen processing and transporting and that PDT activated immune response and cytotoxic T lymphocytes and contributed to the immu-nological recognition.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2010年第7期914-916,共3页
Chinese Journal of Experimental Surgery
基金
基金项目：国家自然科学基金资助项目（30770765）
关键词
胶质瘤
光动力治疗
血卟啉单甲醚
Glioma Photodynamic therapy Hematoporphyrin monomethyl ether
作者简介
通信作者：李方成，Email：sjwklfc@yahoo．com．cn
