摘要
分别以聚马来松香己二醇酯(PMHE)、马来松香丙烯酸乙二醇酯聚合物(PMAGE)和马来松香丙烯酸乙二醇酯-丙烯酸共聚物[Poly(MAGE-AA)]为载体,以Y3+为桥键配离子固定化糖化酶,并测定了固定化糖化酶的性能。结果表明,Poly(MAGE-AA)Y En固定化酶的性能较好,最适温度为50℃,比游离酶高出10℃;最适pH值为5.01;重复使用5次后,活性为1888.00 U.g-1,说明固定化糖化酶的稳定性增强。
Immobilized glucoamylase were prepared by using polymer of maleated rosin acid-hexandiol ester（PMHE）,copolymer of maleated rosin acid-acrylic acid-glycol ester-acrylic acid [Poly（MAGE-AA）] and polymer of maleated rosin acid-acrylic acid-glycol ester（PMAGE） as carriers and the method of rare earth ion coordination.The performances of the immobilized enzymes were determined.The result showed that the highest activity was observed for the Poly（MAGE-AA）YEn.The optimal temperature and pH value was 50℃ and 5.01,respectively.The immobilization enzyme activity was 1888.00 U·g-1 after used for 5 cycles.It proved that the stability of immobilized glucoamylase was increased.
出处
《化学与生物工程》
CAS
2010年第6期62-65,共4页
Chemistry & Bioengineering
基金
广西科学研究与技术开发基金资助项目(桂科攻08395003-9)
关键词
松香基功能高分子
糖化酶
固定化酶
稀土离子
rosin based functional polymer
glucoamylase
immobilized enzyme
rare earth ion
作者简介
袁霖（1984-），男，湖南永州人，硕士研究生，研究方向：天然产物开发与应用；
通讯作者：雷福厚，教授。E-mail：leifuhou@163．com。
