摘要
目的通过对类风湿关节炎(rheumatoid arthritis,RA)患者血清抗血管内皮细胞抗体(Anti endothelial cell anti-bodies,AECA)、血浆血管内皮生长因子(Vascular endothelial growth factor,VEGF)和白细胞介素-17(interleukin-17,IL-17)的检测,以探讨AECA、VEGFI、L-17在RA患者发病、病情进展中的相关性及其内在联系。方法采用间接免疫荧光法(IIF)和双抗体夹心ELISA法检测86例RA、45例骨关节炎(OA)、30例健康对照AECA的阳性率和VEGFI、L-17水平,VEGFI、L-17水平与红细胞沉降率(ESR)、超敏C反应蛋白(hs-CRP)、类风湿因子(RF)等指标进行相关性分析。结果 RA患者AECA阳性率为8.1%,高于OA患者的阳性率2.2%和健康对对照的阳性率0%(P<0.05);RA活动期AECA阳性率为16.7%,高于RA缓解期的阳性率3.6%(P<0.05);RA患者血浆IL-17和VEGF水平显著高于OA组和健康对照组(P<0.05);RA患者活动期血浆IL-17和VEGF水平明显高于RA缓解组及健康对照组(P<0.01);RA患者AE-CA滴度、血浆IL-17、VEGF水平与ESR、hs-CRP、RF的指标呈正相关。结论 AECA、VEGFI、L-17三者与RA的发病、病情活动存在一定的关系,IL-17、VEGF水平变化及AECA的滴度可作为临床观察RA病情活动、判断疗效及预后等方面的参考指标。
Objective To investigate the clinical significance of plasma Anti endothelial cell antibodies（AECA）, vascular endothelial growth factor （VEGF） and interleukin-17 （IL-17） in patients with rheumatoid arthritis.nethods AECA,VEGF and IL-17 were detected in 86 cases of rheumatoid arthritis, 45 cases of osteoarthritis （OA） and 30 healthy controls by indirect immanofluorescence（IIF） and by double antibody sandwich enzyme-hnked immunosorbent assay（ ELISA）. The correlation analysis was done among above results and erythrocyte sedimentation rate （ESR）, high-sensitivity C-reactive protein （hs-CRP）, rheumatoid factor （RF） for correlation! analysis. Results The positive rate of AECA in patients with RA （8.1%） was much higher than that in patients with OA （2.2 % ） and in the normal group（0 % ） （ P 〈 0.05）. The plasma level of IL- 17 and VEGF of RA patients were significantly higher than that of OA patients and the normal control （P 〈 0.05）;plasma level of IL-17 and VEGF of active RA patients were significantly higher than that of RA remission group and healthy control group （ P 〈 0.01 ）, but there was no significant difference between RA remission group and healthy control group（ P 〉 0.05） ;The titer of AECA, plasma IL-17 and VEGF of RA patients are positively correlated with ESR, hs-CRP, RF. Conclusion AECA, VEGF and IL-17 arc correlated with the pathogenesis and activity of RA. they can be used as important indicators for the disease activity, therapeutic effect, prognosis of patients with RA.
出处
《中国实验诊断学》
北大核心
2010年第6期907-910,共4页
Chinese Journal of Laboratory Diagnosis
基金
江西省教育厅资助项目(项目编号:GJJ09099)
作者简介
樊有龙（1973-），男，副教授，副主任技师，主要从事分子和细胞免疫学研究。
通讯作者
