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水貂肠炎病毒的分离及鉴定 被引量:1

Isolation and identification of mink enteritis virus
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摘要 应用细胞培养技术,从2006年山东某貂场送检的水貂病料中分离出1株水貂病毒,经PCR及VP2测序分析证明为水貂肠炎病毒(ZYL-MEV-1);动物感染试验表明是一株强毒株,培养1~20代细胞培养物对猪的红细胞血凝结果表明具有低血凝性,与2009年在大连地区流行的水貂肠炎病毒VP2的基因测序相比较同源率100%,提示此分离株可能为我国目前水貂肠炎病毒的主要流行株。 We apply the cell culture technique to isolate a mink virus from the mink specimens in a mink farm of Shandong in 2006.The virus was identified as mink enteritis virus (ZYL-MVE-1) by VP2 gene PCR amplification and sequencing analysis.A virulence of this virus was characterized by animal infection tests,and porcine red blood cell hemagglutination results in the 1-20th cell cultures show a low hemagglutinating ability.Bsaed on the 100% homology in VP2 gene of virus to the virus strain prevailed in Dalian district 2009,we identified this isolated virus is a major epidemic MEV strain in China.
出处 《中国兽医学报》 CAS CSCD 北大核心 2010年第6期744-747,共4页 Chinese Journal of Veterinary Science
基金 中国博士后基金东北林业大学林学博士后流动站(74224)
关键词 水貂 水貂病毒性肠炎 分离鉴定 mink mink enteritis virus isolation identification
作者简介 谭婷婷(1984-),女,满族,硕士研究生。 通讯作者,E—mail:zhangynlng68@hotmail.com
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