摘要
目的:对疑似甲型H1N1流感患者标本进行病毒核酸检测确诊,探讨实时荧光定量PCR检测方法在流行性感冒检测诊断中的意义。方法:采用WHO标准实时荧光定量PCR方法对2009年6月～12月萧山地区采集的436份疑似甲型H1N1流感患者咽拭子标本进行病毒核酸检测,并对甲型H1N1流感病毒、甲1型流感病毒、甲3型流感病毒和乙型流感病毒进行快速分型。结果:436份咽拭子标本中有144份呈甲型H1N1流感病毒核酸阳性,阳性率33.03%,另检出甲1型流感病毒15份,甲3型流感病毒30份,没有检出乙型流感病毒。结论:实时荧光定量PCR方法操作方便、耗时短、特异性强、灵敏度高,可作为甲型H1N1流感疫情可靠的快速诊断方法。
Objective：To apply a method of real-time fluorescent quantitative PCR for detecting sample from patients with fever and to examine its utility for rapid detection of swine-origin influenza A（H1N1） virus.Methods：A total of 436 oral swab samples from suspected influenza outbreak patients in xiaoshan during the months of June to December in 2009 were detected by real-time fluorescent quantitative PCR method of WHO standards.The samples were subjected to screen in population of influenza virus with influenza A1,A3 and B viruses.Results：Total of 436 oral swab samples,144（33.03%） were positive for swine-origin influenza A（H1N1） virus when tested by real-time fluorescent quantitative PCR,and 15 were positive for influenza A1 virus,30 were potisive for influenza A3 virus,and influenza B virus was not detected.Conclusion：Real-time fluorescent quantitative PCR is an ideal method for detecting of Swine-origin influenza A（H1N1） virus with convenience,good specificity and high sensitivity.
出处
《中国卫生检验杂志》
CAS
2010年第6期1394-1396,共3页
Chinese Journal of Health Laboratory Technology
作者简介
陈棋炯（1983-），男，硕士，主要从事微生物检验工作。
