摘要
目的:观察胡桃醌对内皮细胞系EAHY.926细胞活性和迁移性、大鼠主动脉微血管样结构和鸡胚尿囊膜血管生成的影响。方法:不同浓度胡桃醌作用于EAHY.926细胞株48 h后MTT法检测细胞存活率。大鼠主动脉无血清培养,加入100、50、25、12.5μmol/L浓度的胡桃醌,观察其对微血管结构的影响。选用7日龄鸡胚进行鸡胚绒毛尿囊膜血管生成实验。结果:200、100、50μmol/L浓度胡桃醌对EAHY.926细胞活性有明显的抑制作用(P<0.05),IC_(50)为122.848μmol/L;25、12.5μmol/L的胡桃醌促进EAHY.926细胞株的生长(P<0.05)。100、50、25μmol/L的胡桃醌均可抑制大鼠主动脉微血管形成(P<0.01),12.5μmol/L的胡桃醌可明显减少内皮细胞的爬行数量,延缓其爬行速度(P<0.01)。200、100、50、25μmol/L胡桃醌对鸡胚绒毛尿囊膜已存在的血管有不同程度的刺激作用,出现不同程度的充血、出血,最终凝血,且呈剂量依赖性;而12.5μmol/L的胡桃醌作用区域无新生血管形成。结论:胡桃醌具有抗血管生成作用。
AIM：To investigate the effect of Juglone on the growth and migration of endothelial cell line EAHY.926,the microvessel struc- ture of rat aorta and chick chorioallantoic membrane angiogenesis to provide pre-experimental data for the research of the mechanism of Juglo- ne in preventing tumor angiogenesis factor. METHODS：The effect of a series of concentration of Juglone on the growth of EAHY.926 were measured by MTT.Serum-free culture of the rat aorta was conducted,with 100μmol/L, 50μmol/L,25μmol/L and 12.5μmol/L dosages of Juglone added,to observe its effect on the microvessel structure.7-day-old chick embryo were elected for experimental angiogenesis model. RESULTS：We found that 200μmol/L,100μmol/L and 50μmol/L dosages of Juglone could effectively inhibit the proliferation of EAHY. 926 in a dose-dependent manner（P〈0.05）, with an IC_（50） of 122.848μmol/L according to the results of MTT.While 25μmol/L,12.5μmol/L and 6.25μmol/L dosages of Juglone could pro- mote growth of EAHY.926（P〈0.05）.100μmol/L,50μmol/L and 25μmol/L dosages of Juglone could completely inhibit the formation of new vessels,and 12.5μmol/L dosages of Juglone could reduce the number of endothelial cells.200μmol/L,100μmol/L,50μmol/L and 25μmol/L Juglone had vascular stimulation,hemolyzation, agglutination as a dose-dependent manner.12.5μmol/L Juglone could inhibit angiogenesis. CONCLUSION：Juglone can inhibit the formation of new vessels.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2010年第4期403-410,共8页
Chinese Journal of Clinical Pharmacology and Therapeutics
作者简介
陈丽，女，讲师，研究方向：比较医学。Tel：0551-6167715 E-mail：yaligj@126．com
顾为望，通信作者，男，教授，研究方向：转基因动物制作、比较医学研究、实验动物新品种培育。Tel：021-61648043 E-mail：guww100@163．com
