摘要
目的系统研究蒙古鸦葱的抗肿瘤活性成分。方法使用硅胶H、Sephadex LH-20、ODS和HPLC分离、纯化,根据理化性质和光谱分析鉴定化合物的结构。采用MTT法和SRB法进行活性跟踪和单体化合物的抗肿瘤活性筛选。结果分离鉴定了15个三萜类化合物,分别为羽扇豆醇(1)、白桦脂醇(2)、白桦脂酸(3)、乙酰羽扇豆醇(4)、23Z-3β-acetoxyeupha-7,23-diene-25-ol(5)、达玛二烯醇乙酸酯(6)、齐墩果烷乙酸酯(7)、蒲公英甾醇(8)、蒲公英甾醇乙酸酯(9)、伪蒲公英甾醇(10)、伪蒲公英甾醇乙酸酯(11)、α-乙酰香树脂醇(12)、达玛-24-烯-3β-十四酰氧基-20S-醇(13)、大戟烷-8,24-二烯-3-醇乙酸酯(14)和multiflorenylacetate(15)。结论 1～15均为首次从蒙古鸦葱中分离得到,其中2,3,5～15为首次从该属中分离得到。药理实验表明,在50mg.L-1质量浓度下,化合物1～5对人肺癌细胞A-549、14对人肝癌细胞株Bel-7402显示有意义的细胞毒活性。
OBJECTIVE To study the anti-tumor active constituents of Scorzonera mongolica Maxim..METHODS The chemical components were isolated by silica gel H,Sephadex LH-20 column chromatography and semi-preparative HPLC.Structures were elucidated on the basis of physicochemical properities and spectral data.The anti-tumor experiment in vitro,the MTT and SRB methods were used to screen constituents of S.mongolica Maxim..RESULTS 15 Triterpenes were isolated and identified as lupeol （1）,betulin （2）,betulinic acid （3）,lupeo acetate （4）,23Z-3β-acetoxyeupha-7,23-diene-25-ol （5）,dammaradienyl acetate （6）,pulcherryl acetate （7）,taraxasterol （8）,taraxasteryl acetate （9）,ψ-taraxasterol （10）,ψ-taraxasteryl acetate （11）,α-amyrin acetate （12）,dammar-24-ene-3,20-diol,3β-tetradecanoate （13）,butyrospermyl acetate （14） and multiflorenyl acetate （15）,respectively.CONCLUSION Compound 1-15 were obtained from the plant for the first time and compound 2,3,5-15 were obtained from the genus Scorzonera for the first time.Compound 1-5 demonstrated significant inhibitory effects on A-549 cancer cells and compound 14 showed also strong cytotoxity against Bel-7402 cancer cells at the concentration of 50 mg·L^-1.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2010年第10期727-732,共6页
Chinese Pharmaceutical Journal
基金
国家海洋局908专项(908-01-ST12)
浙江省自然科学基金(Y2080579)
浙江海洋学院重大项目(21135030107)
关键词
蒙古鸦葱
化学成分
抗肿瘤活性
Scorzonera mongolica maxim.
chemical constituents
anti-tumor activity
作者简介
王斌，男，博士，讲师研究方向：海洋药物 Tel：（0580）2555005 E—mail：binwantg4159@hotmail．com
