摘要
目的评价明胶-白芨胶／丹参材料组织相容性，探讨丹参浓度对材料促进损伤组织血管再生作用的影响。方法以明胶和白芨胶为基质材料，复合丹参提取液，制备成多孔材料。175只大鼠随机（随机数字法）为5组，每组35只。四个试验组大鼠皮下依次植入含丹参浓度为1，2，4，5mL／100g的材料，对照组植入单纯明胶-白芨胶多孔材料。术后1d，3d，7d，14d，21d，28d和56d，每组随机选5只大鼠处死。将含周围组织的材料取出，制成病理切片，常规HE染色，镜下观察组织相容性。将术后1d，3d，7d，14d的切片标本行VEGF抗体免疫组化染色，镜下观察比较。结果材料约8周完全降解，组织切片未显示异常反应。免疫组化染色结果显示，材料内丹参浓度约为2mL／100g时，促进血管再生效果最佳。结论明胶-白芨胶／丹参多孔材料具有良好的组织相容性，丹参在材料内促进组织血管再生的适宜浓度约为2mL／100g。
Objiective To evaluate the histocompatibility of gelatin-bletilla/Salvia-mihiorrhiza i material and to discuss the appropriate concentration of salvia-miltiorrhiza in the material. Method Salvia-rnihiorrhiza together with gelatin and bletilla was used to make a kind of porous membranes. A total of 175 rats were divided randomly（random number） into 5 groups （ n = 35 in each）. The porous membranes containing salvia-miltiorrhiza material in different concentrations of 1, 2, 4, and 5 mL/100 g were implanted subcutaneously into the rats of the four experimental groups, respectively. The geiatin-bletilla material without salvia-miltiorrhiza was implanted into the rats of control group. In 1, 3, 7, 14, 21, 28 and 56 days after implant, the materials were taken out of the bodies of the rats, separately. The material implanted for 1, 3, 7 and 14 days were observed with immunabistochemistry. Results In the area of implant, no noticeable inflammation or other was found. The implanted materials were degraded and entirely absorbed in vivo in 8 weeks. The salvia-miltiorrhiza in concentration of 2 mL/100 g in vivo promoted the angiogenesis most significantly. Conclusions The gelatin-bletilla/salvia-mihiorrhiza material has excellent histocompatibility and the appropriate concentration of salvia-miltiorrhiza in material is 2 mL/100 g.
出处
《中华急诊医学杂志》
CAS
CSCD
北大核心
2010年第5期466-469,共4页
Chinese Journal of Emergency Medicine
基金
基金项目：2008年度湖北省教育厅重大项目（7213081601）,2007年度湖北教育厅中青年项目（Q200716005）
作者简介
通信作者：蔡林，Email：guke3224@yahoo．com．cn
