摘要
目的:探讨一次大强度运动对骨骼肌收缩蛋白降解和26S蛋白酶体活性的影响。方法:36只雄性Sprague-Dawley大鼠随机分为6组:安静对照组、运动0.5小时组、运动1小时组、运动1小时后1小时组、运动1小时后2小时组、运动1小时后6小时组,每组6只。运动方式采用一次大强度跑台运动,速度为25m/min,坡度为5%,运动时间分别为0.5小时和1小时。运动后按每组规定时间取材大鼠腓肠肌,观察其3-甲基组氨基酸(3-MH)含量、26S蛋白酶体活性和26S蛋白酶体C2亚基mRNA表达的变化。结果:(1)运动(0.5小时和1小时)后即刻和运动1小时后6小时大鼠腓肠肌3-MH含量显著高于安静对照组(P<0.01),其中运动0.5小时即刻值最高。(2)运动0.5小时即刻、运动1小时后6小时大鼠腓肠肌26S蛋白酶体活性显著高于安静对照组(P<0.05)。(3)运动1小时后6小时大鼠腓肠肌26S蛋白酶体C2亚基mRNA表达显著高于安静对照组(P<0.01),其它时间点却低于安静对照组。结论:运动后26S蛋白酶体活性增高可能促使骨骼肌收缩蛋白降解增强,其活性受亚基基因调控。
Objective To explore the effects of an acute exercise on the contractile protein degradation and 26Sproteasome activity in skeletal muscle. Methods Thirty-six male Sprague-Dawley rats were randomly divided into 6groups,each group consisted of 6 rats. They were killed respectively at rest,immediately after half an hour ofexercise（running on the treadmill at 25m/min,5% grade）,and 1 hour,2h and 6h after one hour exercise. The 3-methylhistidine（3-MH） content,26S proteasome activity and C2 subunit mRNA expression in rat gastrocnemiusmuscle were detected. Results （1）3-MH content increased immediately and 6 hours after exercise,and reached itspeak level immediately after half an hour of exercise （P〈0.01）. （2）26S proteasome activity increased immediatelyafter half an hour of exercise and 6 hours after exercise（P〈0.05）.（3） Expression of 26S proteasome C2 subunitmRNA was lower than baseline during and after exercise,but increased sharply 6 hours after exercise （P〈0.01）.Conclusion The increase in 26S proteasome activity regulated by subunits gene after exercise could enhance skeletalmuscle contractile protein degradation.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2010年第3期305-308,共4页
Chinese Journal of Sports Medicine
基金
国家自然科学基金项目(30971411)
温州医学院科研启动基金(QTJ09018)
关键词
大强度运动
骨骼肌
收缩蛋白降解
26S蛋白酶体活性
acute exercise
skeletal muscle
contractile protein degradation
26S proteasome activity
作者简介
通信作者：王瑞元，Email：wangry@vip．sina．com；Tel：010—62989978
