摘要
目的:建立高效液相色谱(HPLC)法测定化妆品中抗生素和防腐剂的检测方法。方法:采用高效液相色谱(HPLC)法,以甲醇-0.01 mol/L磷酸二氢钠溶液(pH=4.35)为流动相,采用梯度洗脱方式,同时测定化妆品中抗生素和防腐剂9种物质。结果:各物质在1μg/ml～200μg/ml范围内呈良好线性关系(r>0.998),平均回收率93.9%～109.0%,精密度良好RSD<3%。结论:方法灵敏度高,准确度好,操作简单,实用性强,可用于化妆品中抗生素和防腐剂的检测。
Objective：To develop a HPLC method for determination of antibiotics and preservatives in cosmetics.Methods：By using the HPLC method,mobile phase was methanol-0.01 mol/L NaH2PO4（pH=4.35）and gradient elution was used to determine hydroquinone,phenol,antibiotics and preservatives in cosmetics.Results：They showed good linearity within the range of 1 μg/ml～200 μg/ml（r0.998）,average recovery was 93.9%～109.0% and RSD3%.Conclusion：The HPLC method is simple,sensitive,and accurate and can be applied to determination of antibiotics and preservatives in cosmetic preparations.
出处
《中国卫生检验杂志》
CAS
2010年第5期996-997,1024,共3页
Chinese Journal of Health Laboratory Technology
作者简介
于光（1974-），男，本科，主管技师，主要从事理化检验工作。
