摘要
目的:凝胶渗透色谱法测定胶原肽分子质量及其分布。方法:采用三根TSK gel G2000 SWXL(7.8 mm×30 cm,Tosoh)色谱柱串联,流动相为乙腈-水-三氟乙酸溶液(10:90:0.1,v/v/v);流速:0.8 ml/min;检测波长:220 nm;柱温:30℃。结果:在分子质量149 Da～13700 Da之间建立了标准曲线lgMr=-0.126t+7.228,相关系数r=0.994,方法精密度(RSD)小于5.97%。结论:应用本法测定胶原肽分子质量及其分布具有操作简单、精密度高、结果可靠、易于推广的优点。
Objective：To establish a method of gel permeation chromatography to determine molecular weight and its distribution of collagen peptide.Methods：Three TSK gel G2000 SWXL（7.8 mm×30 cm,Tosoh） columns in series was equilibrated with mobile phase composed of acetonitrile/water/trifluoroacetic acid（10：90：0.1,v/v/v） at a flow rate of 0.8 ml/min,detection was performed at 220 nm,and the column temperature was set as 30℃.Results：The relationship curve（lgMr=-0.126t＋7.228） between the logarithm of molecular weight and retention time was obtained in the molecular weight range of 149 Da to 13700 Da.The relative standard deviation（RSD） of the method were less than 5.97%.Conclusion：The method has advantages of simplicity,preciseness,accuracy and easiness.
出处
《中国卫生检验杂志》
CAS
2010年第5期968-969,973,共3页
Chinese Journal of Health Laboratory Technology
基金
浙江省科技厅重大资助项目(2007F10031)
浙江省分析测试计划(2008F70021)
关键词
凝胶渗透色谱
胶原肽
分子质量
分子质量分布
Gel permeation chromatography
Collagen peptide
Molecular weight
Molecular weight distribution
作者简介
贾建萍（1975-），女，助理研究员，主要从事食品生物技术及食品分析工作。
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