摘要
本研究旨在探讨IgA和IgG分泌细胞在断奶仔猪胃肠道不同部位的分布特点和规律。选取8头断奶24 d的仔猪,采集胃底部、胃体部、胃幽门部、十二指肠、空肠、回肠和结肠组织样品,应用免疫组织化学方法检测胃肠道中IgA和IgG分泌细胞的变化。结果显示:IgA和IgG分泌细胞在胃肠道中具有类似的分布特点和规律,即2种分泌细胞主要分布在黏膜固有层中,其数量沿着胃肠道呈现先增加后减少的趋势,其中IgA和IgG分泌细胞在胃幽门部和小肠分布最多。结果表明仔猪的胃幽门部和小肠前段可能是发挥黏膜免疫反应和抵御外界病原微生物入侵的重要位点。
This experiment was conducted to study the distribution characteristics and patterns of IgA and IgG secreting cells in the different sites of the gastrointestinal tract of the weaning piglets.Tissue samples of gastric fundus,gastric body,gastric pylorus,duodenum,jejunum,ileum and colon were collected from eight piglets weaned for 24 days.These tissue samples were studied by immunohistochemistry methods for detecting the changes of IgA and IgG secreting cells in the gastrointestinal tract.The results showed that the distribution of IgA and IgG secreting cells along the gastrointestinal tract had similar distribution tendency and patterns.IgA and IgG secreting cells were mainly distributed in the lamina propria,and the numbers of the two sorts of secreting cells were firstly increasing and then decreasing along the gastrointestinal tract.In addition,IgA and IgG secreting cells were mostly distributed in the gastric pylorus and the small intestine.These results demonstrated that the gastric pylorus and the anterior segment of the small intestine of piglets may be the important sites of playing the mucosal immune reactions and protecting against the pathogenic microorganism.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2010年第5期615-620,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家公益性农业行业科研专项(200803020)
教育部博士点基金(B200606)
关键词
仔猪
胃肠道
IgA分泌细胞
IgG分泌细胞
piglet
gastrointestinal tract
IgA secreting cells
IgG secreting cells
作者简介
作者简介：车传燕（1980-），女，山东烟台人，博士，主要从事微生物与免疫方向的研究，Tel：025—84395817，E—mail；ccyanl980@163．com
通讯作者：杨倩，教授，E—mail：zxbyq@njau．edu．cn
