摘要
为了进一步探索辽宁绒山羊卵母细胞体外成熟的方法,本试验以屠宰场绒山羊卵巢为材料,采用抽吸法收集直径大于2 mm卵泡的卵母细胞,研究卵丘细胞对卵母细胞体外成熟和孤雌发育的影响。试验1,将一部分COCs经机械吹打脱除卵丘细胞成为机械裸卵(DOs),然后以4种方式培养,即COCs单独培养、DOs与COCs共培养(DOs(COCs))、DOs与卵丘颗粒细胞共培养(DOs(CCs)),以及DOs单独培养。试验2,根据包裹卵母细胞的卵丘细胞的完整性分为3组,有3层卵丘细胞紧密包围的卵母细胞复合体COCs3,有1-3层卵丘细胞包围的卵母细胞COCs1-3,无卵丘细胞包围的裸露卵母细胞NOs,3组各自单独培养。结果表明:COCs组的成熟率、孤雌卵裂率和囊胚率最高,分别为83.25%、41.75%和29.25%,卵丘细胞的存在有利于卵母细胞体外成熟和随后的发育,COCs3组成熟率、孤雌卵裂率和囊胚率分别为83.25%、42.50%和29.75%,显著高于COCs1-3和NOs组,卵丘细胞的完整性也影响着卵母细胞的体外发育,自然裸卵已失去体外发育能力。
To explore the method of in vitro maturation of Liaoning Cashmere goats oocytes,this experiment was carried out to study effects of cumulus cells on in vitro maturation and parthenogenetic development of oocytes.The oocytes were recovered directly from the oocytes of follicles,2 mm in diameter.Experiment 1,DOs（denuded oocytes） were obtained from COCs freed of whole CCs,and then the oocytes were cultured by the following four ways：（1）COCs were cultured alone;（2） DOs were co-cultured with COCs（DOs（COCs））;（3） DOs were co-cultured with CCs（DOs（CCs））;（4） DOs were cultured alone.Experiment 2,the effect of different layers of cumulus cells on the maturative ability and parthenogenetic development of oocytes were evaluated.These oocytes were divided into COCs3,COCs1-3 and NOs according to the layers of cumulus cells.We found that there was significant difference in maturation and cleavage rates between ooctyes invested by different layers of cumulus cells,and the oocytes enclosed with more layers of cumulus cells,both maturation and cleavage rates were higher than oocytes enclosed with fewer layers of cumulus cells.The DOs（COCs） was better than DOs（CCs）,and the NOs was a kind of degenerate ooctyes.Our findings would be helpful to the choice of recipient oocytes for embryonic technologies which will need high quality oocytes.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2010年第5期543-548,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
辽宁农业职业技术学院重点项目
常年长绒型辽宁绒山羊新品系选肓扩繁及产业化示范(2009BADA5B02)
关键词
卵丘细胞
卵母细胞
体外成熟
孤雌发育
辽宁绒山羊
cumulus cells
oocytes
in vitro maturation
parthenogenetic development
Liaoning Cashmere goats
作者简介
作者简介：田长永（1978-），男，辽宁普兰店人，硕士，主要从事畜禽繁育技术研究，E—mail：dkswtcy@163．com.
通讯作者：马泽芳，E—mail：mazefang@163．com.
