摘要
河岸葡萄叶的带正电荷过氧化物酶（ｃａｔｉｏｎｉｃ ｐｅｒｏｘｉｄａｓｅ， ＣＰＯＤ） 可受６ＢＡ 诱导，但盐、Ｈ２Ｏ２ 或Ｆｅ２＋＋ Ｈ２Ｏ２ 均使叶片ＣＰＯＤ 活性明显下降，而高浓度的无机盐明显刺激纯ＣＰＯＤ 活性增加。在以愈创木酚为底物时ＣＰＯＤ 最适ｐＨ 为４ ．６０ ～５ ．７５ ，对Ｈ２Ｏ２ 的表观Ｖｍａｘ 和Ｋｍ 值分别为１１０ Ｕ／ｍｇ 蛋白和１ ．１５ｍ ｍｏｌ／Ｌ。
A cationic peroxidase (CPOD) was purified from leaves of Vitis vulpina L, and some of its characteristics were analyzed. The results showed that the cationic peroxidase could be induced by 6 benzylaminopurine. It also inhibited markedly by salinity, H 2O 2 or Fe 2+ +H 2O 2, in vivo . But the purified CPOD was stimulated significantly by high concentrations of inorganic salts. The optimum pH of the CPOD activity was between 4.60 and 5.75 with guaiacol as substract. The apparent V max and K m were 110 U/mg protein and H 2O 2 1.15 mmol/L, respectively.
基金
河北大学基金
关键词
过氧化物酶
6-BA
无机盐
葡萄
cationic peroxidase, 6 benylaminopurine, inorganic salt, Vitis vulpina
