摘要
目的探讨子宫内膜癌(EC)中肿瘤相关基因DNA启动子区CpG甲基化谱(CIMP)状态及意义。方法应用甲基化特异性聚合酶链反应技术,分别检测35例EC组织、15例癌旁配对组织及22例正常子宫内膜组织中P14、P16、ER、RASSF1A及COX-2基因启动子区甲基化修饰状况,并分析CIMP的频率及与各基因甲基化的关系。结果 EC组织中甲基化频率从P16的37%至P14的57%不等,癌旁配对组织中由P16的27%至P14的60%不等。正常子宫内膜组织中仅有2例ER、COX-2和4例RASSF1A甲基化。46%(17/35)的EC和47%(7/15)的癌旁组织为CIMP+(P=0.574),正常组织中未见CIMP+。在CIMP+的EC中,CIMP+与P16和COX-2的甲基化相关(P=0.001)。结论多基因同时甲基化在EC及癌旁组织频率较高,P16和COX-2的甲基化与CIMP+状态相关,CIMP+可能是子宫内膜癌癌变的早期事件。
Objective To investigate the frequency and significance of CpG island methylator phenotype（CIMP）in endometrial cancer（EC）.Methods The promoter methylation status of P14,P16,ER,RASSF1A and COX-2 was examined in 35 cancer tissue,15 matched adjacent nontumor tissues,and 22 normal endometrial tissue by methylation-specific PCR.The correlation of CIMP＋ frequency and methylation of genes was analyzed.Results The methylation frequency of promoters for 5 genes in cancer tissue ranged from 37% for P16 to 57% for P14,and ranged from 27% for P16 to 60% for P14 in matched adjacent nontumor tissues.CIMP＋ was frequent in cancer and adjacent nontumor tissue（46% and 47%,respectively,P=0.574）and absent in normal tissue.Moreover,CIMP＋ was significantly correlated with methylation of P16 and COX-2（γ=0.673 and 0.662,respectively,P=0.001）.Conclusion CIMP＋ is an important and frequent epigenetic event in EC tissue or adjacent nontumor tissue and may be a biomarker for predicting early carcinogenesis.Methylation of COX-2 or P16 was related with CIMP＋ in this cancer.
出处
《实用癌症杂志》
2010年第3期247-251,共5页
The Practical Journal of Cancer
基金
广东省自然科学基金项目(9151503102000039)
广东省医药卫生科学研究基金项目(A2009430)
作者简介
通讯作者：张庆英
