摘要
目的探讨芹菜素对氧糖剥夺(OGD)损伤后复氧复糖(OGD/R)小胶质细胞中白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)产生的影响。方法将原代培养的小胶质细胞随机分为正常组、二甲基亚砜(DMSO)组及芹菜素(10、25、50μmol/L)组,其中DMSO组和芹菜素组小胶质细胞进行OGD8h,之后加入相应药物培养基,观察24h后,收集细胞上清,用双抗夹心酶联免疫吸附法检测上清中IL-1β、TNF-α含量。结果24h后DMSO组细胞上清中IL-1β、TNF-α含量增多(P<0.01)。芹菜素组TNF-α、IL-1β分泌量明显低于DMSO组。芹菜素可以抑制OGD后小胶质细胞的炎症反应,且呈剂量依赖性。结论芹菜素可以通过减少OGD/R小胶质细胞中IL-1β、TNF-α的产生而发挥神经保护作用。
Objective To study the neuroprotective effect of apigenin on microglia which was exposed to oxygen glucose deprivation and reperfusion （OGD/R）, which was characterized by its influence on IL-1β and TNF-α expression. Methods Primary microglial cultures were prepared from newborn rat brain. The purity of isolated cells were identified by GSA-IB4. The cells were randomized into 5 groups：normal group, DMSO group and apigenin-treated groups （10, 25, 50 μmol/L）. The cells of DMSO group and apigenin-treated group were exposed to 8 h of OGD and 24 h of reperfusion in the presence or absence of apigenin at a range of concentrations. Culture supernatants were collected and IL-1β and TNF-α were detected by ELISA assay. Results The expression of IL-1β and TNF-α were significantly higher in DMSO group （P〈0.01）, however, apigenin concentration-dependently suppressed IL-1β and TNF-α production of microglial. Conclusion Apigenin may play a neuroprotective effect which was related with depression of IL-1β and TNF-α production in OGD/R microglia.
出处
《中国中医药信息杂志》
CAS
CSCD
2010年第3期26-28,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
温州市科技局国际合作项目(H20070034)
作者简介
通讯作者：陈翔，E-mail：chenxiangnj2005@yahoo．com．cn
