摘要
背景:研究表明造成高分子导管相关感染的原因是介入材料在体内停留一段时间容易引起病原菌在其表面吸附、定殖甚至形成菌膜,成为潜在的感染源。抑制菌膜的形成可显著改善高分子导管相关型感染的发生。目的:观察抗感染聚乙烯材料的抗菌效果及其对表面菌膜形成的抑制作用。设计、时间及地点:单一样本观察,于2008-06/2009-05在中科院理化所工程塑料国家工程研究中心完成,其中抗菌检测于2009-03在解放军总参谋部总医院完成。材料:有机抗菌剂为2,4,4’-三氯-2’-羟基二苯醚;无机抗菌剂为IONPURE银系抗菌剂;低密聚乙烯。方法:低密度聚乙烯与有机和无机两种抗菌剂混合后先挤出造粒,然后注塑成片。主要观察指标:①用活菌平板计数法测定材料的抗菌性能。②平板超声波法及扫描电镜判断材料表面菌膜的形成情况。结果:①两种改性抗菌材料对大肠杆菌和金黄色葡萄球菌都有良好的抗菌效果,抗菌率达到99.9%。②细菌菌膜(菌膜)的形成主要包括黏附、繁殖和成熟3个阶段,添加有机抗菌剂的试样在菌膜形成前就可以显著抑制细菌在其表面生长,从而抑制菌膜形成,扫描电镜结果表明其表面未见菌膜形成。空白试样和无机抗菌试样表面均有大量细菌和细胞外基质黏附。结论:有机抗菌聚乙烯对大肠杆菌和金黄色葡萄球菌有良好的抑菌作用,可抑制细菌在材料表面形成菌膜,为防治医用导管相关感染提供了新的途径。
BACKGROUND：Studies have demonstrated that intervention material retention for a period of time in vivo causes pathogenic bacteria surface attachment,proliferation even forming biofilm to become potential source of high polymer catheter-associated infection.Inhibition of biofilm formation significantly reduces infection.OBJECTIVE：To evaluate the antimicrobial effect of anti-infective polyethylene material and its inhibiting effect to biofilm on the surface.DESIGN,TIME AND SETTING：Single sample observation was performed at the National Engineering Research Center for Engineering Plastics,Technical Institute of Physics and Chemistry,Chinese Academy of Sciences from June 2008 to May 2009,and the antimicrobial test was performed at the General Hospital of General Staff Headquarters of PLA in March 2009.MATERIALS：Organic antibacterial agent2,4,4＇-trichloro-2＇-hydroxydiphenyl ether;IONPURE Silver-Inorganic anti-microbial reagent;low-density polyethylene （LDPE）.METHODS：LDPE was respectively blended with organic antibacterial agent and inorganic antibacterial agent,and the LDPE slice was prepared by injection molding process.MAIN OUTCOME MEASURES：The antimicrobial efficacy of anti-infective polyethylene was determined by the plate counting method.The ultrasonic-plate method and scanning electron microscopy were introduced to observe the biofilm formation.RESULTS：①Both of modified LDPE samples exhibited excellent antimicrobial performance against S.aureus and E.coli.The antimicrobial rate was 99.9%.②The formation of biofilm could be described as a three-stage process：adhesion,proliferation and biofilm maturation.The propagation of microbe was found to be depressed remarkably on the surfaces of modified samples with organic antibacterial agent,and the formation of biofilm was prevented.The observation of SEM confirmed that no biofilm was formed on the surfaces of PE added organic antibacterial agent.The results showed that a large amount of bacteria and extracellular polysaccharide matrix adhered on the surfaces of PE added inorganic antibacterial agent and untreated PE.CONCLUSION：Anti-infective polyethylene added organic antibacterial agent possesses excellent antimicrobial performance against S.aureus and E.coli,and it can inhibit the formation of biofilm on the surfaces,which provides a promising approach to prevent catheter-related infections.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第51期10016-10020,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
中国科学院知识创新工程重大项目"科技助残计划项目"支持(KGCX2-YW-608)~~
作者简介
李红梅，女，1969年生，河北省沧州市人，汉族，2001年解放军军医进修学院毕业，博士，副主任医师，主要从事呼吸病相关临床和基础研究。jhji@263．net
通讯作者：季君晖，博士，博士生导师，研究员，中国科学院理化技术研究所，北京市100190
