摘要
以日本圆叶海棠初代培养获得的茎段为外植体,采用正交和随机区组设计培养基进行继代和生根培养。建立了日本圆叶海棠试管苗快繁体系,并优化获得其适宜继代的培养基MS+IBA 0.2 mg/L+6-BA0.3 mg/L+蔗糖3%+琼脂0.55%,适宜的生根培养基为1/2MS+IAA 0.5 mg/L+蔗糖2%+琼脂0.55%。研究表明,MS基本培养基附加低浓度水平的6-BA与IBA的激素组合,有利于丛生芽的诱导,1/2MS基本培养基附加低浓度的IAA,有利于根的诱导,NAA与IBA不适用于生根。生根苗的移栽可采用温室自然光过渡法炼苗后,以蛭石为基质的营养钵移栽法。
Taken the original cultured stem sections of Japanese Malus prunifolia var.ringo as explants,the mediums for subculture and rooting culture were designed by using the orthogonal and the randomized block experiments.Then,the rapid propagation system of tube seedling of Japanese Malus prunifoliavar.ringo was well established.The results showed that the suitable medium for subculture was composed of MS ＋ IBA 0.2 mg/L ＋ 6-BA 0.3 mg/L ＋ sucrose 3% ＋ agar 0.55%,and medium for rooting culture was composed of 1/2MS ＋ IAA 0.5 mg/L ＋ sucrose 2% ＋ agar 0.55%.It indicated that the addition of the low concentration of 6-BA and IBA to MS basic medium is advantageous to the induction of axillary bud.Moreover,1/2MS basic medium combined with the low concentration of IAA was useful to the rooting induction.However,NAA and IAA were not suitable for the rooting culture.Transplanting of rooting seedlings may use the method of greenhouse natural light transition to build up the seedling after,use the nutrition earthen bowl transplants in greenhouse take the vermiculite as the matrix.
出处
《西北农业学报》
CAS
CSCD
北大核心
2010年第2期183-188,共6页
Acta Agriculturae Boreali-occidentalis Sinica
基金
陕西省教育厅重点实验室项目资助
作者简介
陈宗礼（1954-），男，陕西扶风人，教授，硕士生导师，从事遗传学教学与植物遗传育种研究。Email：Zongli_chen@yahoo.com.cn
