摘要
"梅桥"大豆是安徽省淮北地区农家菜用大豆品种,长年的连续种植导致纯度比较混杂,产量明显下降,利用SSR分子标记技术对92单株"梅桥"大豆种质纯度进行鉴定,以期为梅桥大豆的提纯复壮提供理论依据。结果表明:采用改良的CTAB方法,可以提取高质量的大豆基因组DNA;在优化SSR-PCR反应体系的基础上,筛选出8对SSR引物,平均每个引物得到条带数10.6个,条带的多态性频率为70.9%,梅桥大豆的种质纯度为38.04%。
＂ Meiqiao＂ is a vegetable soybean with good quality in Huaibei Anhui Province, as result of continuous cultivation, the purity and yield reduced seriously. In this article, 92 plants were selected to identify the purity using SSR molecular markers so that it could provide theoretical guide for purification and rejuvenation of ＂Meiqiao＂ Soybean. The results showed that the high quality soybean genomic DNA was extracted by improved CTAB method, under optimization of SSR-PCR reaction system, eight SSR primers were selected and 10.6 bands were obtained from each one, the rate of polymorphic bands wasT0.9% , the purity of ＂Meiqiao＂ Soybean germplasm was 38.04%.
出处
《大豆科学》
CAS
CSCD
北大核心
2010年第2期210-214,共5页
Soybean Science
基金
安徽高校省级自然科学研究重点资助项目(KJ2008A089)
作者简介
盛新颖（1982-），女，硕士，研究方向为大豆分子育种。E-mail：lingjun19850420@126.com。
通讯作者：林毅，教授，博士生导师。E-mail：linyiahau@126．com。
