摘要
目的观察人尿激肽原酶(HUK)对大鼠脑缺血再灌注(I/R)后大脑皮质缺血灶周围区Caspase-3、凋亡诱导因子(AIF)的时相表达及对神经细胞凋亡的影响。方法将Wistar大鼠随机分为假手术组、模型组、HUK干预组,应用线栓法建立大鼠大脑中动脉阻塞(MCAO)再灌注模型。假手术组于术后,模型组、HUK干预组于缺血2h后再灌注6h、24h、48h、72h,应用免疫组化技术和原位末端标记法(TUNEL)检测不同时相各组缺血灶周围脑区Caspase-3、AIF和TUNEL阳性细胞的表达。结果大鼠I/R后在缺血灶周围区各个时间点均可见到Caspase-3、AIF和TUNEL阳性细胞的表达,HUK组与模型组相比较,两组Caspase-3、AIF和TUNEL阳性细胞的表达趋势基本一致,Caspase-3、AIF和TUNEL的表达高峰均在I/R后24h。HUK组各时间点Caspase-3、AIF和TUNEL阳性细胞的光密度值较模型组明显降低(均为P<0.05)。结论HUK对大鼠脑缺血再灌注损伤有保护作用,其作用机制可能与HUK抑制Caspase-3、AIF的表达,减轻迟发性神经元的凋亡有关。
Objective To investigate the effect of human urinary kallidinogenase（HUK）on the expression of caspase-3,apoptosis-inducing factor（AIF）and neuronal apoptosis in perifocal areas of cerebra after ischemia-reperfusion（I/R）in rats. Methods Wistar rats were randomly divided into sham group,model group and HUK group. The middle cerebral artery occlusion/reperfusion（MCAO/R）model was established by thread ligation. After operation in sham group,2h ischemia and 6h,24h,48h and 72h reperfusion in model and HUK group,the expression of caspase-3 and AIF positive cells and neuronal apoptosis in the ischemic cortex were detected by immunohistochemistry and in situ apoptotic detection（TUNEL method）. Results The caspase-3,AIF and TUNEL positive neurons expressed in perifocal areas at different time points after I/R. The trends of expressed caspase-3,AIF and TUNEL positive neurons in HUK group and model group were basi-coincidence. The peak of expression was all at 24h in perifocal areas after I/R. The optical density value of caspase-3,AIF and TUNEL positive neurons in HUK group at each time were significantly decreased compared with model groups（P0.05）. Conclusion HUK plays protective function for the cerebral tissues after I/R by reducing the expression of caspase-3,AIF and tardive neuronal apoptosis in posse.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2010年第3期233-236,共4页
Journal of Apoplexy and Nervous Diseases
关键词
人尿激肽原酶
细胞凋亡
CASPASE-3
AIF
脑缺血再灌注
Human urinary kallidinogenase
Cell apoptosis
Caspase-3
Apoptosis inducing factor（AIF）
Cerebral ischemia-reperfusion
作者简介
杨金升，通讯作者
