摘要
目的:探讨溶栓颗粒对TNF-α损伤血管内皮细胞ICAM-1表达的影响。方法:用TNF-α(40ng/mL)损伤人脐静脉内皮细胞(HUVEC)造模,用含大、中、小剂量溶栓颗粒的大鼠血清干预受损HUVEC,应用HE染色观察细胞形态变化,免疫组化法观察血管细胞间黏附分子-1(ICAM-1)的表达。结果:TNF-α可以造成HUVEC损伤,表现细胞数量下降,皱缩、变形,分裂相细胞明显增加。受损细胞的ICAM-1表达明显增多。含大、中剂量溶栓颗粒的大鼠血清能减少ICAM-1表达,减少细胞有丝分裂。结论:溶栓颗粒可以减少因炎性介质TNF-α增加而受损的血管内皮细胞ICAM-1表达,或许可以减缓动脉粥样硬化形成;溶栓颗粒减少血管内皮细胞分裂增殖,对稳定动脉粥样斑块稳定性或许有积极作用。
Objective： To study the effect of Rongshuan Grain on Injury Caused by Tumor Necrosis Factor α TNF -α
） of the human umbilical vein endothelial cells（HUVEC） on the expression of intercellular adhesion molecule -1 （ICAM -1 ）. Methods： Use the TNF- ct（40ng/mL）to damage the HUVEC, use the serum of Rats that contain different doses＇Rongshuan Grain to interference the damaged HUVEC,and observe the morphological changes by HE staining and the expression of ICAM - 1 by immunohistechemical method. Results ： TNF - ct can damage the HUVEC, manifestations included that the cell number is decreased, the cell shape change to crimple and the division cells are increased. The damaged HUVEC~ expression of ICAM - 1 is increased. The serum of Rats that contain high dose and moderate dose of Rongshuan Grain can reduce the expression of ICAM - 1, and reduce the number of division cells. Conclusion： Rang- shuan Grain can reduce the expression of ICAM - 1 on the damaged HUVEC by TNF - ct. This function maybe decrease the formation of atherosclerosis. Rongshuan Grain can reduce the number of division cells of he damaged HUVEC, this function maybe effective on the stabihzation of atheromatous plaque.
出处
《中华中医药学刊》
CAS
2010年第4期873-875,共3页
Chinese Archives of Traditional Chinese Medicine
基金
青岛市中医科研计划资助项目(2006-zyy009)
关键词
溶栓颗粒
细胞间黏附分子-l
人脐静脉内皮细胞
肿瘤坏死因子-Α
rongshuan grain
vascular endothelial growth factor
intercellular adhesion molecule - 1
human umbili- cal vein endothelial cells
tumor necrosis factor α
作者简介
作者简介：臧运华（1975-），男，山东胶南人，主治医师，博士，研究方向：中西医结合防治脑血管病。
