摘要
目的:观察补肾复方逆转压力负荷增加大鼠心肌纤维化的作用。方法:采用腹主动脉狭窄所致压力负荷增加大鼠左室重构模型,将雄性SD大鼠60只随机分为假手术组、模型组、卡托普利组、补肾复方大剂量组、补肾复方小剂量组,观察用药4周后左室心肌病理形态胶原染色、左室心肌间质超微结构、左室心肌Ⅰ型胶原免疫组织化学染色、左室心肌Ⅲ型胶原免疫组织化学染色等指标的改变。结果:模型组Ⅰ型胶原的平均光密度与假手术组比显著升高(P<0.01),卡托普利组、补肾复方大剂量组、小剂量组的Ⅰ型胶原平均光密度均较模型组显著下降(P<0.01)。模型组Ⅲ型胶原的平均光密度与假手术组比显著升高(P<0.01),卡托普利组、补肾复方大剂量组、小剂量组的Ⅲ型胶原平均光密度均较模型组显著下降(P<0.01)。左室心肌病理形态胶原染色、左室心肌间质超微结构的改变与Ⅰ型胶原和Ⅲ型胶原的改变基本一致。结论:补肾复方具有与卡托普利基本一致的逆转心肌纤维化的作用。
Objective ： To observe the effects of Bu Shen Prescription on regressing myocardial fibrosis in rat with pres- sure overload. Methods ： The model of left ventricular remodeling in rat with pressure overload by abdominal aortic con- striction was used. 60 SD male rats were randomly divided into five groups： sham operation group, model group, eapto- pril group, Bu Shen Prescription high dosage group and low dosage group. After treatment 4 weeks, it was observed that the changes of left ventricular myocardium collagen pathological coloring, left ventricular interstitial cardiac tissue ultrami- crostructure, left ventricular cardiac tissue collagen type I immunohistochemistry coloring and collagen type Ⅲ immuno- histochemistry coloring. Results ： The average optic density of left ventricular cardiac tissue type I collagen and type Ⅲ collagen of model group were increased obviously than that of sham operation group （ P 〈 0.01 ）. The average optic den- sity of left ventricular cardiac tissue type I collagen and type Ⅲ collagen of captopril group, Bu Shen Prescription high dosage group and low dosage group were lower than those of model group significantly（ P 〈 0. 01 ）. The changes of left ventricular myocardium collagen pathological coloring and left ventricular interstitial cardiac tissue ultramicrostructure were consistent with those of type I collagen and type Ⅲ collagen. Conclusion ： Bu Shen Fu Prescription and captopril had similar effects on regressing myocardial fibrosis.
出处
《中华中医药学刊》
CAS
2010年第4期677-681,共5页
Chinese Archives of Traditional Chinese Medicine
基金
南京军区南京总医院科研基金资助项目(Q2008042)
关键词
心肌纤维化
压力负荷
I型和Ⅲ型胶原
补肾复方
myocardial fibrosis
pressure overload
collagen type I and Ⅲ
Bu Shen Prescription
作者简介
作者简介：蔡辉（1959-），男，江苏启东人，主任医师、教授，博士研究生导师，中医学内科学博士，中西医结合博士后，主要从事中西医结合基础与临床研究。
