摘要
采用岛津高效液相色谱仪,紫外检测器,CBM-102工作站,分析柱为DiamonsilC18(150mm×4.6mm,5m)。血清样品甲醇萃取,风干后,配制成100:l水溶液进样检测。所建立的检测条件为:流动相乙腈-水(18:82 V/V),流速0.5 ml/min;咖啡因为内标,终浓度8mg/ml;检测波长200 nm。结果显示,环磷酰胺标准品线性方程为:线性范围为10-200 mg/l。表明实验中所建立的方法色谱峰分离较好。
In ooder to develop a High Performance Liquid Chromatography （HPLC） method for determining the concentration ofCyclophosphamide （CP） in the plasma of the immunosuppressed mice, the Shimadu HPLC system was used. The analytical column was a diamonsil C 18 column, with the detective UV wavelength of200nm. Acetonitrille and water with the rate of 18 ： 82 （V/V） and with the flow rate of 0.5ml/min was used in isocritic mobile phase. The plasma samples were collected, and then were deposited down the protein by methanol. The remains was air-dried and dissolved with 100？ 1 water. Caffeine was added as the internal standard with a final concentration of 8mg/ml. The results showed that the liner equation was, It determinated that the chromatography was consistent with the parameters used here.
出处
《湖南农机（学术版）》
2010年第1期80-82,共3页
Hunnan Agricultural Machinery
关键词
环磷酰胺
HPLC
小鼠
HPLC, Cyclophosphamide, Mice, Immunosuppres
作者简介
作者简介：李素华（1980-），女，湖北十堰人，副教授，博士，研究方向：动物普通病分子机理研究。
